COSMOSIL
COSMOSIL
COSMOGEL
COSMOGEL
Reversed Phase High Performance Liquid Chromatography
COSMOSIL PNAP Packed Column
COSMOSIL PNAP is a Reversed Phase HPLC Column with Naphthalene bounded silica packing material. Naphthalethyl group has two aromatic rings and has strong P-P interaction. This column offers improved separation of
compounds such as positional isomers that are difficult to separate with Alkyl group bonded materials.
Features of COSMOSIL PNAP
- Naphthalene bonded silica packing material
- Stronger P-P interactions than Phenyl column
Material characteristics
Packing material
Silica gel
COSMOSIL PNAP
high purity spherical porous silica
Average particle size
Average pore size
5Mm
approx. 120Έ
Specific surface area
Stationary phase
approx. 300m2/g
naphtylethyl group
Bonded type
Main interaction
monomeric type
P-P interaction
End-capping treatment
Carbon content
treated
Naphtylethyl group
Si
CH3
H3C
approx. 11%
Comparison with Phenyl columns
COSMOSIL PNAP with two aromatic rings has storonger P-P interactions than Phenyl column which has one aromatic
ring.
Ph
Si
H3C
PNAP
PE-MS
Si
CH3
H3C
Si
CH3
H3C
CH3
The difference of interaction between
benzene and nitrobenzene
P㪄P㩷㫀㫅㫋㪼㫉㪸㪺㫋㫀㫆㫅
Stationary phase
Strong P-P interaction
PNAP
Weak㩷P-P interaction㩷
Ph, PE-MS
H
H
H
C
㱜NAP
H
C
C
C
C
H
H
Strong
NO2
1.00
Benzene
1.20
1.40
㱍(Nitrobenzene/Benzene)
1.60
Nitrobenzene
C
H
C
H
H
Sample
Ph
C
C
C
H
C
C
C
C
Weak
PE-MS
H
H
C
H
C
H
Application data
Position isomers of Tolunitrile
CH3
5C18-MSΤ
5PE-MS
NAP
(40%Methanol)
(40%Methanol)
(50%Methanol)
CH3
CH3
CN
CN
CN
m-
o-
po-
p-
po-
m-
Column size
Flow rate
Temperature
Detection
Sample
m-
o-
m-
p-
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
o-Tolunitrile (2.0 g)
m-Tolunitrile (2.0 g)
p-Tolunitrile ‫ޓޓ‬
(2.0 g) ‫ޓ‬
Position isomers of Phthalonitrile
5C18-MSΤ
5PE-MS
(30%Methanol)
(40%Methanol)
NAP
CN
CN
CN
CN
(50%Methanol)
CN
CN
o-isomer
o-, p-
m-isomer
p-isomer
ppm-
o- mo- m-
Column size
Flow rate
Temperature
Detection
Sample
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
Phthalonitrile
(0.3 g)
Isophthalonitrile (3.0 g)
Terephthalonitrile (0.15
‫ ޓޓ‬g) ‫ޓ‬
Tocopherol
R1
5C18-MSΤ
5PE-MS
NAP
(95%Methanol)
(90%Methanol)
(90%Methanol)
HO
R2
O
R3
R1
Me
Me
H
R2
Me
H
Me
H
R3
Me
Me
Me
Me
Column size
Flow rate
Temperature
Detection
Sample
H
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 295nm
Tocopheril ( ฀ ฀ ฀ )
(1.25 g each)‫ޓޓ‬
Adrenal cortex hormone
5C18-MSΤ
5PE-MS
PNAP
(50%Methanol)
(50%Methanol)
(60%Methanol)
OH
HO
1.Prednisolone
OH
O
2.Hydrocortisone
Column size
Flow rate
Temperature
Detection
Sample
HO
OH
O
HO
O
O
O
O
O
HO
HO
3.Cortisone
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
1. Prednisolone (0.33Mg)
2. Hydrocortisone (0.33Mg)
3. Cortisone ‫(ޓޓ‬0.33Mg) ‫ޓ‬
Isoflavone
5C18-MS-II
5PE-MS
PNAP
Column size
Mobile phase
4.6mmI.D. x 150mm
A:Acetonitrile/20mmol/l
Phosphate Buffer(pH2.5)= 10/90
B:Acetonitrile/20mmol/l
Phosphate Buffer(pH2.5)= 50/50
B‫ޓ‬0ψ100%/20min linear gradient
1.0ml/min
30oC
UV 260nm
1. Daidzin (0.05Mg)
2. Glycitin (0.05Mg)
3. Genistin (0.05Mg)
4. Daidzein (0.05Mg)
5. Glycitein (0.075Mg)
‫ޓޓ‬
6. Genistein (0.025Mg)
‫ޓޓ‬
Flow rate
Temperature
Detection
Sample
Berberine
5C18-MSΤ
5PE-MS
O
OMe
㩷P㪥㪘㪧
O
OMe
N+
MeO
OMe
Cl-
1.Palmatine Chloride
N+
MeO
OMe
Cl-
2.Berberine Hydrochloride
Column size 4.6mmI.D. x 150mm
Mobile phase 5C18-MS-II : Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 40/60
5PE-MS
: Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 60/40
ǸNAP‫ ޓޓ‬: Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 80/20
Flow rate
1.0ml/min
Temperature 30oC
Detection
UV 254nm
Sample
1. Palmatine Chloride (0.2Mg)
2. Berberine Hydrochloride (0.2Mg)
‫ޓޓ‬
Futher separation
COSMOSIL PYE is a packed column with pyrene bonded silica packing material. It has stronger P-P interaction than
PNAP since pyrene has four atromatic rings.
Isoflavone
R
5C18-MSΤ
PNAP
㪧㪰㪜
(98%Methanol)
(90%Methanol)
(95%Methanol)
HO
R=
1.Cholesterol
2.Stigmasterol
Si
3.Campesterol
4.Sitosterol
Column size
Flow rate
Temperature
Detection
Sample
4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 210nm
1. Cholesterol
2. Stigmasterol
3. Campesterol
4. Sitosterol
‫ޓޓ‬
Ordering information
Product name
COSMOSIL PNAP
Packed Column
Size
1.0mmI.D. x 150mm
1.0mmI.D. x 250mm
2.0mmI.D. x 150mm
2.0mmI.D. x 250mm
3.0mmI.D. x 150mm
3.0mmI.D. x 250mm
4.6mmI.D. x 50mm
4.6mmI.D. x 100mm
4.6mmI.D. x 150mm
4.6mmI.D. x 250mm
10.0mmI.D. x 150mm
10.0mmI.D. x 250mm
20.0mmI.D. x 150mm
20.0mmI.D. x 250mm
28.0mmI.D. x 250mm
Code No.
08076-61
08077-51
08078-41
08079-31
08080-91
08081-81
08083-61
08084-51
08085-41
08086-31
08088-11
08089-01
08092-41
08093-31
08095-11
Product name
COSMOSIL PNAP
Guard Column
Size
4.6mmI.D. x 10mm
10.0mmI.D. x 20mm
20.0mmI.D. x 20mm
20.0mmI.D. x 50mm
28.0mmI.D. x 50mm
Code No.
08082-71
08087-21
08090-61
08091-51
08094-21
Other size may be available. Please enquire!!
For research use only, not intended for diagnistic or drug use.
NACALAI TESQUE, INC.
Hichrom Limited
Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855, JAPAN
The Markham Centre, Station Road
TEL:1+81-75-251-1730
Reading, Berks, RG7 4PE
FAX:Theale,
+81-75-251-1763
Tel: 0118 930 3660
Web site: http://www.nacalai.com
Fax: 0118 932 3484
E-mail:
info.intl@nacalai.co.jp
Web
site: www.hichrom.co.uk
Email: sales@hichrom.co.uk