Attachment G.
Research Organisms – Vertebrates, Invertebrates, or Plants
General Project Title:
YES
N/A
Does your research involve the use of animals and/or plants in conjunction with previously
mentioned materials in Attachment A, B, C, or E?
If “Not Applicable”, please skip Attachment G and proceed to the next applicable
attachment. If “YES” continue below.
Are the transgenic animals or plants used or created as part of the research? If yes,
complete Attachment A.
i.
Research Organism Summary
Please check all that apply.
We will be introducing materials into research organisms. Please describe below.
We will be introducing recombinant DNA into research organisms. Please describe below. Complete Attachment A.
We will be introducing infectious materials into research organisms. Please describe below. Complete Attachment B.
We will be introducing OTCC into research organisms. Please describe below. Complete Attachment C.
We will be introducing toxic or hazardous materials into research organisms. Please describe below. Complete
Attachment D.
We will be creating transgenic animals. Please describe below.
We will be purchasing transgenic animals. Please list supplier and type of transgenic animal.
We will be transferring transgenic animals. Please describe below and list prospective recipients.
We will be breeding transgenic animals. Please describe below.
ABSL1
ABSL2
We will be creating transgenic plants. Please describe below.
We will be purchasing transgenic plants.
We will be transferring transgenic plants.
PBSL 1
PBSL 2
USDA-APHIS Permit required Type/number:
USDA-APHIS Notification Number:
We will be performing safety tests or screening for pathogenic or infectious agents before or after inoculation into
animals. Please describe below.
We will be using types (or modification of types) that may be perceived to significantly diverge from standard practices
in our field. Please explain below.
We will be performing (or directing others to perform) practices that may be perceived to significantly diverge from
standard practices in our field. Please explain below.
Provide additional information from the checked box above. Briefly summarize the overall goal and specific objectives of
the work with these materials. (e.g. mouse used for pharmaceutical study)
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Attachment G of
General BUA Application
ii.
Animal Research Organism
If not applicable, check here
.
If vertebrate species involved, list IACUC protocol ID:
Research Organism
(Genus, species, strain)
Recipient of:
rDNA
Construct
(germ line or
somatic?)
Vector
Microbe
Yes
No
Yes
No
Yes
No
iii.
Chem
-ical
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Adminis
tration
Route
Transgenic/Genetically Modified Animal
Gene Modification
Insert Delete/Silence
iv.
OTCC
Gene Name
Species of Animal/Strain
Max.
Vol.
Route of
Shedding/
Excretion &
Interval
If not applicable, check here
Gene Source
or Species
Encode for
If not applicable, check here
Biological
Agent or
rDNA
.
Phenotypic results, expected tissue
expression
ABSL2 Containment
Species of Animal/Strain
Max.
Conc.
Admi
nister
ed
.
Containment/Work Practice/Locations
Prior to procedures
# of Animals
Infectious Agent
During Procedures
Post Procedures
Duration of Infectivity*
*Attach Literature or Reference for the infectious agents’ duration of infectivity for the research organism.
v.
Plant Research Organism
Species of Plant
Transgenic
Yes
Yes
Yes
vi.
No
No
No
If not applicable, check here
USDA-APHIS
Permit/Notification
(Type, Number)
Yes
Yes
Yes
Noxious
Weed (US
or CA)
No
No
No
Yes
Yes
Yes
Transgenic/Genetically Modified Plant
Method of Reproduction
(self, wind pollinator, insect pollinator, human
intervention required)
No
No
No
If not applicable, check here
Recombinants will have special growth requirements:
Yes
Describe requirements:
Recombinants are expected to be more pathogenic:
Yes
Describe why it is expected to be more pathogenic:
No
No
a. Transformation Method
Agrobacterium tumefaciens (all tumorigenic DNA removed)
Particle bombardment
Vacuum infiltration
Other:
b. Inserted or Deleted/Silenced Gene Information
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Rev. 12/14
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.
Attachment G of
General BUA Application
.
Gene Modification
Insert Delete/Silence
Gene Name
Gene Source
or Species
Encode for
Phenotypic results
c. Characterization of Inserted Gene
Pharmaceutical
Pesticide, microbial pesticide, other plant-incorporated protectant
Pesticide resistance
Pathogen, pest, or herbicide resistance
Selectable marker genes that will not be removed
Other:
vii.
Plant Containment
If not applicable, check here
Greenhouse
PBSL
Growth Chamber
PBSL
(i.e. 1, 2, 3)
Pollen
Seeds
Whole plants
Tissue Culture Use
PBSL
(i.e. 1, 2, 3)
(i.e. 1, 2, 3)
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Motile macrorganisms insects,
nematodes
Yes
No
Yes
No
Yes
No
Microorganisms
Yes
No
Yes
No
Yes
No
viii.
Field Release
.
If not applicable, check here
.
Describe method for field release and the impact it may have on the normal flora or fauna.
ix.
Transportation
If not applicable, check here
.
Describe transportation method for transport. Include any methods to prevent release during transport:
Indicate transportation locations:
x.
Methods to Minimize Dissemination and Accidental Release If not applicable, check here
Removal of flower and seed heads
Harvest prior to sexual maturity
Cloaking of flowers to prevent seed and pollen dispersal
Use of male sterile lines
Keeping distance between infected plants and a susceptible host
Choosing season/chronological timing of experiment to prevent cross contamination of susceptible plants
Removal of vectors for insect borne transmission
Use of plants genetically disabled for survival in the wild
Describe environmental impact if accidentally released. Include methods to prevent release if not indicated above:
Submission
Submit completed Attachment G with General BUA Application to the IBC at Gateway
Center 3519D, MC 1933 and an electronic copy to ibc@mail.sdsu.edu.
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Attachment G of
General BUA Application
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