Name:_________________________________ Date:____________ Period:________
Replication
1. What is the difference between the lagging strand and the leading strand?
Describe the function of the following enzymes involved in replication
2. DNA helicase –
3. DNA ligase –
4. DNA polymerase –
5. RNA primase –
6. The template strand of a piece of DNA being replicated reads: 5'-ATAGGCCGT-3'. A partially
synthesized Okazaki fragment is 5'CCTA3'. If the next fragment is four bases long, what is
its first base?
DNA Repair
7. This segment of DNA has undergone a mutation in which
six nucleotides have been deleted. A repair enzyme
would replace them. Which series of bases will
complete the strand of DNA?
8. A particular sequence of parent DNA has five purine bases and two pyrimidine bases. According
to base-pairing rules, which of the following sequences could be formed during replication?
a.
two cytosine, three adenine, two thymine
b.
two cytosine, three adenine, two uracil
c.
three adenine, two thymine, one guanine, one cytosine
d.
three adenine, two guanine, two cytosine
9. Explain how replication occurs, include enzymes involved and describe the semi-conservative
nature of DNA replication.