PANWAT 2002 Meeting Agenda
September 19th and 20th
EMSL Auditorium, Pacific Northwest National Laboratory
Richland, Washington
Proteomics Short Course, Thursday, September 19th, 1:00 – 5:15 p.m.
Chairpersons: Rick Zangar and Joel Pounds, Pacific Northwest National Laboratory.
The proteomics short course is designed to present an overview of new developments in proteomics and related
fields. The course will be started off by Dr. Richard Smith, who leads a large proteomics research effort at
PNNL. Other speakers will cover a variety of related topics. Presentations should contain an overview of the
field suitable for the novice as well as highlight more specialized research interests.
#1
1:00 – 2:00
Comprehensive Proteomics: Is it Possible and is it Needed?
Richard D. Smith
Pacific Northwest National Laboratory, Richland, WA
#2
2:00 – 2:30
MALDI TOF/TOFTM Mass Spectrometry
Douglas F. Barofsky
Department of Chemistry, Oregon State University, Corvallis, OR
#3
2:30 – 3:00
Bacterial Identification by MALDI Mass Spectrometry
Karen L. Wahl, Sharon C. Wunschel, Nancy B. Valentine, Catherine E. Petersen, David B. Wunschel, Mark T.
Kingsley, and Kristin H. Jarman,
Pacific Northwest National Laboratory, Richland, WA 99352
3:00 – 3:15
Break
#4
3:15 - 3:45
A Protein Microarray ELISA for Measuring Cancer Biomarkers.
Susan Varnum, Ronald Woodbury, Richard Zangar
Pacific Northwest National Laboratory, Richland, WA
#5
3:45 – 4:15
Specific Single-Chain Antibodies Produced in a Yeast Host
Mike Feldhaus
Pacific Northwest National Laboratory, Richland, WA
#6
4:15 – 4:45
Bioinformatics: An Introduction to Tools and Resources
Ken Auberry
Pacific Northwest National Laboratory, Richland, WA
#7
4:45 – 5:15
Characterization of Membrane and Shed Proteins by Mass Spectrometry
David L Springer, Deanna L. Auberry, Josh N. Adkins, David S. Wunschel, Jane M. Feldhaus, Jon H. Wahl,
and Karin D. Rodland.
Pacific Northwest National Laboratory, Richland, WA
Poster Session and Mixer, Thursday, September 19th, 5:15 – 7:00 p.m.
EMSL Rooms 1075 and 1077.
Platform Sessions, Friday, September 20th
“Cellular and Organismal Responses to Oxidative Stress”
First Morning Session: Student Presentations
Chairperson: Terry Kavanagh, University of Washington
#8
8:00 – 8:30 Effects of Phytochemicals on Aflatoxin B1-Mediated Genotoxicity in HepG2 Cells
KM Bradley, K Gross-Steinmeyer, DL Eaton.
University of Washington, Seattle WA.
#9
8:30 – 9:00 Hydroxyl Radical is Produced in Mitochondria only when Electron Transport is
Inhibited.
Melissa M. Mackey and Carin Thomas.
Department of Chemistry MS-7539, Central Washington University, Ellensburg, WA
#10
9:00 – 9:30 Association between a Trinucleotide Repeat Polymorphism in the Glutamate
Cysteine Ligase Catalytic Subunit Gene and Lung Disease in Adult Cystic Fibrosis Patients.
J Shao, E F. McKone†, C L. Keener, C A. Shephard, F M. Farin, M L. Aitken† and T J. Kavanagh.
Department of Environmental Health, Center for Ecogenetics and Environmental Health and †Division of
Pulmonary and Critical Care Medicine, University of Washington, Seattle WA
Break 9:30 – 9:45
Second Morning Session:
Chairperson: ????
#11 9:45 – 10:25 Oxidative Stress, Glutathione and Cell Signaling
Donald J. Reed
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331
#12 10:25-11:05 To be announced.
Marc Fariss
Washington State University, Pullman, WA
#13 11:05-11:45 Effects of Tea Antioxidants In Vitro and in Mouse Models of Colorectal Cancer:
Modulation of Wnt Signaling.
Gayle A. Orner*, Wan-Mohaiza Dashwood*, Qingjie Li*, Mohamed Al-Fageeh*, Niall Tebbutt†, Joan K.
Heath†, Matthias Ernst† and Roderick H. Dashwood*
*Linus Pauling Institute, Oregon State University, Corvallis, OR 97331-6512, USA and †Ludwig Institute for
Cancer Research, Melbourne, Australia.
Lunch
11:45 – 12:35
EMSL 1075/1077
Student Awards
12:35 – 12:45
EMSL Auditorium
Afternoon Sessions:
Chairperson: Joe Beckman, Oregon State University
#14 12:45 – 1:25 Peroxynitrite and Tyrosine Nitration as Mediators of Human Disease.
Joe Beckman
Linus Pauling Institute, Environmental Health Sciences Center, Department of Biochemistry and Biophysics,
Oregon State University.
#15 1:25 – 2:05
To be announced.
Tom Squier
Pacific Northwest National Laboratory
#16 2:05 – 2:45
Biological Implications for Tyrosine Nitration in Muscle
#*
Diana J. Bigelow , Rosa I. Viner&, Victor S. Sharov&, Christian Schoneich&, Todd D. Williams&, and Tanya
V. Knyushko#
#
Pacific Northwest National Laboratory, *Washington State University Tri-Cities, and &University of Kansas
Break
2:45 – 3:00
Chairperson: Tom Weber, Pacific Northwest National Laboratory
#17 3:00 – 3:40
Regulation of Activator Protein-1 (AP-1) by 8-iso-PGE2 in a Thromboxane A2
Receptor-Dependent and -Independent Manner.
Thomas J. Weber and Lye Meng Markillie
Battelle, Molecular Biosciences, Richland, WA 99352
#18 3:40 – 4:20
Gene Regulatory Changes During the Macrophage Inflammatory Response
B.Y. Chin, M. Markillie, L. Kathman, and Brian D. Thrall.
Pacific Northwest National Laboratory
PANWAT Business Meeting:
EMSL Auditorium
4:20 – 4:50
Posters, Attended During Mixer on Thursday, September 19th, 5:15 – 7:00 p.m.
#19
Effects of Dietary Vitamin E on Preneoplastic Colon Lesions and α-Tocopherol Tissue Levels in
Young and Old Rats
J H. Exon, E.H. South, J.G. Zhang and M.W. Fariss
University of Idaho (JHE, EHS), Washington State University (JGZ, MWF)
#20
Altered mRNA Expression of Cytochromes P-450 1A1 and 1A2, NAD(P)H:quinone
oxidoreductase and glutathione-S-transferase A1 by the Phytochemicals Curcumin, 3,3’Diindolylmethane, Isoxanthohumol, 8-Prenylnaringenin, Phenethyl Isothiocyanate and Sulforaphane in
Cultured Human Primary Hepatocytes.
Kerstin Gross-Steinmeyer1, Patricia L. Stapleton1, Fei Liu1, Julia H Tracy1, Sean Quigley1, Theodor K.
Bammler1, Stephen C. Strom2, and David L. Eaton1.
1
University of Washington, Department of Environmental Health, Seattle, USA; 2 University of Pittsburgh,
Department of Pathology, Pittsburgh, USA
#21
Hepatic and Intestinal A-Esterase Metabolism of CPF-Oxon and DZN-Oxon.
Hong Wu, Karl Weitz, Chuck Timchalk, Torka Poet.
Battelle, Pacific Northwest Division, Richland, WA, 99352.
#22
Comparative Study of the Kinetic Interaction of Chlorpyrifos Oxon with Rat Cholinesterases.
Ahmed Kousba, Torka Poet and Charles Timchalk.
Battelle, Pacific Northwest Division, Richland, WA, 99352.
#23
Global Proteomics for Human Blood Serum I: Multidimensional Separation of Peptides Coupled
with Mass Spectrometry
Joshua N. Adkins, Susan M. Varnum, Kenneth J. Auberry, Ronald J. Moore, Nicolas H. Angell, David S.
Wunschel, Richard D. Smith, David L. Springer, and Joel G. Pounds
Molecular Biosciences Department, Pacific Northwest National Laboratory, Richland, WA, 99352
#24
Proteomic profiles of prostate cancer using human tissues
Mamoun Ahram1, Michael J. Flaig1, John W. Gillespie2, Paul H. Duray3, W. Marston Linehan4, David K.
Ornstein5, Hungjun Shu6, Yingming Zhao6, Emanuel F. Petricoin III7§, and Michael R. Emmert-Buck1
1
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, National Cancer Institute,
National Institutes of Health, Bethesda, MD; 2Science Applications International Corporation, National Cancer
Institute, Bethesda, MD; 3Laboratory of Pathology, National Cancer Institute, National Institutes of Health,
Bethesda, MD; 4Urologic Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda,
MD; 5Department of Urology, University of North Carolina, Chapel Hill, NC; 6Department of Biochemistry,
UT Southwestern Medical Center, Dallas, TX; 7Tissue Proteomics Unit, Center for Biologics, Evaluation and
Research, FDA, Bethesda, MD
#25
Suppression of glutathione biosynthesis during TGFβ1-induced hepatocyte apoptosis
Christopher C. Franklin, Maryland E. Rosenfeld, Collin White, Terrance J. Kavanagh and Nelson Fausto
University of Washington, Department of Pathology (CCF, MER, NF), Department of Environmental Health
(CW and TJK).
#26
A proteomics study of glycosylated membrane proteins
Deanna L. Auberry, David S. Wunschel, David L. Springer
Pacific Northwest National Laboratory, Richland, Washington
Student Poster Presentations:
#27
Glutamate Cysteine Ligase Catalytic Subunit Trinucleotide Repeat Polymorphism and Type I
Diabetes.
L. Bekris, C. Shephard, M. Zarghami, F. Farin, W. Griffith, T. Kavanagh, A. Lernmark.
University of Washington, Seattle, WA.
#28
Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on neutrophil-attracting chemokines and
reactive oxygen species (ROS) production in the lungs of influenza A virus infected mice.
Sabine Teske, Josh Neumiller*, B. Paige Lawrence
Pharmacology/Toxicology Graduate Program, *Doctor of Pharmacy Program, College of Pharmacy,
Washington State University, Pullman, WA
Housing:
Attendees must make housing arrangements themselves. Attendees are encouraged to use the new User
Housing Facility (UHF) on the PNNL Campus. Rates are very reasonable and the facility is less than a block
from EMSL, the meeting site. Notably, student/postdoc rates for a private “dorm” room (with a private bath
and shared kitchen/eating area) are $21/night. Larger rooms are also available at reasonable rates. There is a
limited number of rooms available and reservations are on a first-come, first-serve basis. There is not a block of
rooms set aside for the meeting.
User Housing Facility (Onsite housing): Room options and rates can be viewed at
http://www.pnl.gov/uhf/index.html. Reservations can be made online or contacting
Sandra Linfoot
UHF Manager
Phone: 509-372-6736
Fax: 509-372-6738
E-mail: uhf@pnl.gov
Hotels in Richland (generally within a 10 min drive, all are on or near George Washington Way, and easily
accessible.) A hotel map can be located at http://www.visittri-cities.com/map_lodging.shtml.
Red Lion (Hanford House) – 590-946-7611 (Nicer hotel with view of river. Pool and sauna, dining facilities.)
Bali Hai – 509-943-3101 (Nice but presumably less expensive hotel. Pool and sauna.)
Royal Hotel and Casino – 509-946-4121 (used to be Holiday Inn. Pool and sauna, dining facilities.)
Hampton Inn – 509-943-4400 (Nicer hotel with view of river.)
Shiloh Inn – 509-946-4661 (Nice hotel near river. Pool and sauna, dining facilities.)
Map:
A local map to the User Housing Facility can be obtained at http://www.pnl.gov/uhf/images/uhflocation.jpg.
Although not marked as such on the map, the large gray building shown across the street from the UHF is the
EMSL facility, where the meeting will be held.
Abstract Submission:
Abstract deadline is September 3rd, 2002. Send abstracts by email to Rick Zangar (richard.zangar@pnl.gov).
Abstract text should be limited to 250 words. The first author should be the individual giving the presentation.
Platform presentations will be scheduled for 30 – 45 min, including the question and answer period. My
apologies for the inexact time period, but I expect to adjust timing slightly depending upon the number of
presenters. Platform presentations by established investigators should deal with the general theme of the
meeting, cellular and organismal responses to oxidative stress. Platform presentations by students and poster
presentations on any topic are welcome.
When submitting an abstract, please indicate the following information in the cover letter:
1) If the presenter is a student or postdoc.
2) Platform or poster presentation.
Other Activities:
The Tri-Cities’ area offers a selection of golf courses, wineries and other attractions
such as jet boat tours of the White Bluffs on the Columbia River. Information on local attractions can be found
at http://www.visittri-cities.com/welcome.shtml.
PANWAT 2002 Meeting Registration Form
Thursday, Sept. 19th
Expected Attendance:
Friday, Sept. 20th
Name (First, Middle Initial, Last)
Company Name
Department
Street Address
City/State/Zip
Area Code
Phone Number
Fax Number
E-mail address (please include – all communication is expected to be by e-mail)
Please check, as appropriate
Student
Postdoc
Faculty/other
Registration Fees (By September 3rd):
Students
($30)
All Others
($80)
Meals*
$20
Total
*
The fee for meals must be paid by everyone, including students and late-registrants. This fee will cover snacks on Thursday evening
(presented at the poster session), and a continental breakfast and lunch on Friday. Late registration or registration at the meeting will
be possible but the availability of meals for these individuals will depend upon the caterer’s ability to accommodate late registrants.
(There is an inexpensive place to buy lunch in EMSL.)
Method of Payment:
 Check or Money Order #
 American Express (15 digits)
Credit Card #:
Signature:
 Government Purchase Order #
(US Gov’t PO form must be attached)
 Diner’s Club (14 digits)
 Master Card (16 digits)
 Visa (13 or 16 digits)
Expiration Date:
/
Cardholder’s Printed Name:
Return this form with payment to:
Tom Weber, Molecular Biosciences, Battelle, P7-56, P.O. Box 999, Richland, WA 99352
Fax: 509-376-6767. Faxed forms are accepted only if using a credit card.
If registering after September 3rd, please email Tom Weber (thomas.weber@pnl.gov) of your intent to register as soon as possible.
Date received: