COLLEGE OF DENTISTRY
DEPARTMENT OF MAXILLOFACIAL SURGERY
AND
DIAGNOSTIC SCIENCES [MDS]
ORAL BIOLOGY [113 MDS]
Lab Manual
Head Of the Department
Course Director
PRACTICAL: 1
TOPIC: Development
of teeth
Identify Dental Lamina in the diagram. What is dental lamina? Enumerate the physiologic and
morphologic stages of tooth development.
Label the diagram and write about the important features of this stage
BUD stage:
Label the diagram and write about the important features of this stage
Cap Stage:
Label the diagram and write about the important features of this stage
Early bell stage:
Label the diagram and write about the important features of this stage
Advanced Bell Stage
What is Hertwigs epithelial root sheath? Writ about root formation:
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PRACTICAL: 2
TOPIC: Enamel
Which cells of the enamel organ transform (convert) into ameloblasts? Enumerate the stages in
life cycle of an ameloblast:
Maturative stage of life cycle of ameloblasts:
Write about the importance of this stage in amelogenesis:
Enamel Rods and interrods:
What are enamel rods? Label Enamel rods and interrods in the given photograph and write the chemical
composition of enamel
Incremental lines of enamel:
Label the diagrams and write about striae of retzius and perikymata.
Neonatal line:
Label the given diagram. What is neonatal line and in which teeth do you see the neonatal line?
Enamel Tufts: label the diagram. What are enamel tufts?
Enamel lamellae: label the diagram. What are enamel lamellae and what are the types of enamel
lamellae.
Enamel spindles: label the diagram. What are enamel spindles? How are they formed?
Hunter - Schreger bands: what are Hunter – Schreger bands? How are they formed? What are diazones
and parazones? Label the diagram.
Gnarled enamel: what is gnarled enamel? Which part of the crown is it usually seen? What is its clinical
significance?
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Practical :3
Topic: Dentin
Write the chemical composition of dentin. Enumerate various types of dentin. Label the given diagram.
Dentinal tubules: what are dentinal tubules? what is the shape of the dentinal tubules? Label the
diagram
Transverse section of dentin: label the diagram. What is peritubular dentin and intertubular dentin?
Dentinoenamel junction: what is the shape of dentinoenamel junction? What is the importance of the
Dentinoenamel junction.
Interglobular dentin: where is interglobular dentin present? What are Von Kroff fibres?. Label the
diagram.
Incremental lines of dentin: what are the incremental lines of dentin? What are contour lines of owen?
Label the diagram.
Tomes granular layer: what is tomes granular layer? How is it formed? Label the diagram.
Dead tracts: what are dead tract? How are they formed? How do they appear under transmitted light
and reflected light? Label the diagram
Sclerotic dentin: what is sclerotic dentin? How is it formed? Label the diagram.
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PRACTICAL: 4
TOPIC: Dental Pulp
Zones of pulp: what are the different zones of pulp? Enumerate the functions of pulp. Label the
diagrams
Pulp stones:
What are they also known as? How are they formed? What are the types of pulp stones? Label
the diagram.
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PRACTICAL: 5
TOPIC: Cementum
Acellular cementum : Label the following diagram. Write about the various types of cementum.
Cellular Cementum: label the diagram and write the differences between cellular and acellular
cementum.
Cementoenamel junction: label the diagrams. write the clinical significance and formation of all
types of junctions.
a. Sharp junction (butt type junction)
b. Gap junction
Overlap junction:
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PRACTICAL: 6
TOPIC: Periodontal
Ligament
Principal fibres of periodontal ligament: Label the diagram and enumerate the principal fibres of
periodontal ligament. What are sharpeys fibres?
Gingival group of Fibres: Label the diagram. Enumerate gingival group of fibres and write about
the functions of periodontal ligament.
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PRACTICAL: 7
TOPIC: Alveolar
bone:
Define alveolar process. What are the parts of alveolar bone? Label the diagram.
Lamina dura: Label the photograph. What is lamina dura? what is Cribriform plate?
Osteoclasts: What is the function of osteoclasts? What is howships lacunae?
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PRACTICAL: 8
TOPIC: Oral
Mucous Membrane
Non keratinized epithelium: Label the diagram. Enumerate the layers of keratinized and
nonkeratinized epithelium.
Keratinized epithelium: Label the diagram. Write the difference between parakeratinized and
orthokeratinized epithelium
Papillae of tongue: identify the papillae given below and mention their location on the tongue.
Vermillion border of lip: Label the diagram what are the other names of vermillion border?
Taste buds: Where are the taste buds seen?. Enumerate the types of cells seen in taste buds,
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PRACTICAL: 9
TOPIC: Salivary
Glands
Serous salivary gland: label the diagram and write the features of serous acini.
Mucous salivary gland: label the diagram and write the features of mucous acini.
Serous demilune: what are serous demilune and where are they seen?
Myoepithelial cell: identify myoepithelial cell in the given photograph. Enumerate its functions.
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PRACTICAL: 10
TOPIC: Dental
Age Estimation
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
In the given dental cast identify all the teeth present and write their tooth number in FDI system;
mention the last erupted tooth and estimate the dental age of the patient.
Name
University
no.
Submission
date
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Signature