The Protestant Legend
Presented by: Thao Cao
Mentor: Dr. Hiro Nonogaki
Seed Biology Laboratory
The Protestant Legend
http://www.ccmb.res.in/staff/imran/Resources.html
 Food
 Genetic information
 Dispersal unit
http://ucsdnews.ucsd.edu/graphics/images/pod%5B1%5D.gif
http://commons.wikimedia.org/wiki/File:Pumpkin_seeds.jpg
Brassicaceae
Broccoli
Cauliflower
Brussels
Sprouts
Canola
http://www.gettyimages.com/detail/sb10065002w-001/Digital-Vision
http://www.worldofstock.com/closeups/PFO3982.php
http://www.haverford.edu/biology/Johnson/Modelingt
heOrganism/Modeling_the_Organism.htm
Dry
seed
NF-YA3
Expression
High
NF-YA9
Dry
seed
http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi
Low
Down-regulation of NF-YA gene by
microRNA is essential to maintain normal
developmental program of seeds.
MicroRNA
RISC
RISC
RISC
Mutant
Control
Wild type
 Conditional expression
 Chemical
 Alcohol
 Heat Shock
Chemical
Induction
Switch
CsMV
VP16 Activation
GAL4 DNA Binding
Ecdysone Receptor
Promoter
Chemical Induction
(ligand: methoxyfenozide)
Active
Gene of Interest
5XUAS
Promoter
Courtesy of Dr. Roger Beachy, Donald Danforth Plant Science Center
GUS
Promoter
Induced
GUS
Promoter
Uninduced
5 days after Imbibition
Wild type
AGE:GUS
-
-
+
+
Uninduced
Induced
NF-YA3
-
+
mNF-YA3
-
+
Wild type
-
NF-YA9
Actin
+
NF-YA9
-
+
mNF-YA9
-
+
Howard Hughes Medical Institute
Dr. Kevin Ahern
Dr. Hiro Nonogaki
Louis Stokes Alliance for Minority Participation
Natalya Goloviznina
Theresa Nguyen
Dr. Cristina Martinez-Andujar
Mariko Nonogaki
Satoru Yamagishi