PI Name:
Protocol #:
APPENDIX F. ADJUVANT/ANTIGEN USE
1. Indicate what antigen will be used.
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2. Indicate what vehicle/adjuvant will be used:
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3. Initial immunization:
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4. Subsequent immunizations:
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5. Anticipated complications/side effects:
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6. Indicate sites for immunization:
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7. Indicate route of administration:
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8. What is the total and per site injection volume?
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9. What is the frequency/duration of immunization (e.g., 1 injection every 2 weeks for 3
injections)?
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Ascites production. Fluid accumulation associated with ascites/hybridomas should not become greater
than 10% of body weight. Animals must be euthanized if they become moribund or severely dyspneic.
1. Indicate the maximum volume of ascites fluid to be collected per sampling (ml/mouse) and the
method of collection (i.e. skin prep, gauge needed, gravity vs. suction).
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2. Indicate the number of fluid collections and anticipated frequency of collection.
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3. Describe procedures used to care for and monitor the health of animals with ascites and the
point of euthanasia.
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DOR.ORPCC.A.0F - IACUC NP Appx F Adjuvant Antigen
Form updated January 2014
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