Wednesday,)September)5,)2012)
Vanderbilt)Conte)Center)Research)Program)Monthly)Meeting)
U@1202)Learned/Neuroscience)Conference)Room)
!
Welcome)and)Program)Update:)Randy)Blakely!
• New)Conte)Structure)
o 4!projects!
o 5!cores!
o Advisory!boards!!
! External—Susan!Dymecki,!Lou!Muglia,!Miklos!Toth,!one!to!visit!each!year!
! Internal—Danny!Winder,!Karoly!Mirnics!and!Jim!Sutcliffe,!semi!annual!meeting!with!
RB!and!MW!
o Quarterly!Core!Director!meetings!
!
• Goals)of)Projects!!(RB!handout))
)
• Core)Updates)
o Cost!for!Neurochemistry!services!for!projects;!!no!kitty!this!go!cycle,!pay!internal!rates!
directly!from!projects!
o Crawley!and!Lucki!consultants—please!use!!
o New!animal!protocol!in!Physiology!&!Behavior!Core!(Support!by!behavior!core!includes!
update!to!their!protocol,!compared!to!last!Conte!where!individual!PIs!had!their!own!animal!
protocols.!!Gregg!can!elaborate,!but!if!Investigators!need!to!make!changes,!forward!that!
info!to!Gregg,!he’ll!update!the!protocol!and!submit!to!IACUC,!then!notify!PI!when!they!can!
begin!new!experiments).!!Local!PIs!can!use!their!own!protocol,!but!must!add!Gregg!and!
Tommy!to!their!protocol.!
o Note:!!mouse!quarantine!time!is!8!weeks!
o New!Core!at!USC:!!Molecular!Neuroanatomy!
! Juli!Wu!is!managing!director!
! Anddre!Valdivia!is!research!technician,!already!trained!in!immunostaining,!mouse!
transcardial!perfusions,!cryostat!sectioning,!plasmid!extractions!and!
transformations.!!Currently!learning!to!optimize!protocols!and!antibody!testing,!then!
will!focus!on!in!situ!hybridization!
! Will!create!core!tissue!library!
! Will!develop!new!in!situ!hybridization!techniques!to!better!examine!distribution!of!
splice!variants!and!microRNAs!
!
• Budgetary)Review!(i.e.!cuts)!
o Projects!and!cores!cut!15%!
o No!cuts!on!educational!supplement!
!
• Conte)Meetings)at)USC)and)CW)
)
• Outreach)and)Educational)Supplement)!
o Educational!Supplement!includes!
! Summer!Student!Program!(4!students)!
!
!
!
Stipend!support!for!Outreach!
• Gwynne!Davis!
• Justin!Siemann!!
Brain!Matters!expansion!
Public!Lecture!series,!e.g.,!3!per!year!if!external!speakers!invited,!more!if!use!internal!
faculty!
!
!
Program)Support)Review:)Denise)Malone)
• Other!Outreach!
o Installation!at!Perot!Museum’s!Being!Human!Hall!approved!and!moving!forward!
o Development!Office!and!Brain!Matters:!!Foundation!Relations!and!Corporate!Relations!
o Final!progress!report!from!ARRA!Supplement!completed!
o VBI!/!Conte!applied!for!SfN’s!Program!of!the!Year!!
• Website!redesign!!
• 2012/2013!Meeting!Schedule!
• Symposium!Reminder!!
o cofsponsors!are!
! Peabody!College!
! VKC!
! Pharmacology!
! Molecular!Physiology!&!Biophysics!
! Psychiatry!
o Schedule!is!
! 8:00!AM,!Brain!Matters!Tour!
! 9:00!AM,!Laboratory!of!Neurobehavior!Tour!
! 10:00!AM,!Faculty!Meeting!
• Holmes!with!Emeson!
• Ansorge!with!Blakely!
• Lin!with!Jeremy!VVW!
• Gaspar!with!Deneris!
! 11:15!AM,!Lunch!at!University!Club!(Randi!Ulbricht,!Richard!O’Neil,!Jeremy!VVW,!Liz!
Hammock,!Nicole!Baganz,!Ran!Ye)!
! 12:15!PM,!Escorted!to!Law!School!for!Symposium!
• Updates!in!progress!at!OHO!
o New!videos!filmed!and!uploaded!
o In!process!
! Narrations!for!Discovery!
! Transitioning!application!formats!for!Neuroscience!Discovery!to!increase!flexibility!of!
content!and!ease!of!use!for!guests!
! Mark!has!been!talking!to!(Dr.!David!Weintraub),!who!has!three!students!lined!up!to!
assist!with!content!at!Brain!Matters.!!Students!are!juniors!and!seniors!in!the!
Communication!of!Science!and!Technology!program!at!Vanderbilt!and!the!interested!
students!are!either!neuroscience!or!biology!majors,!so!they!should!be!a!great!
resource!to!expand!content.!
!
!
!
Brief)Updates)Cores:!!!
• Bioanalytical!Core!
o Core!Director:!!Ariel!Deutch!
o Core!CofDirector:!!Paul!Gresch!
o Core!Research!Assistant:!!Katia!Belova!
o Neurochemistry!Core!Manager:!!Ray!Johnson!
• Physiology!and!Behavioral!Core!
o Core!Director:!!Doug!McMahon!
o Core!CofDirector:!!Gregg!Stanwood!
o Core!Research!Assistant!Professor:!!Hideki!Iwamoto!
o Core!Research!Assistant:!!Tommy!Saborido!
• Molecular!Neuroanatomy!Core!
o Core!Director:!!Pat!Levitt!
o Core!Manager:!!Juli!Wu!
o Core!Research!Assistant:!!Anddre!Valdivia!
• Biostatistics!and!Informatics!
o Core!Director:!!Bing!Zhang!
o Core!CofDirector:!!Lily!Wang!
o Core!Research!Fellow:!!Jing!Zhu!
!
Brief)Updates)from)Pilot)Projects:))!
• Heidi!Hamm!
• Allison!Lampert/Bruce!Carter!
• Ran!Ye!
!
Brief)Updates)Conte)Investigators:!!
• Evan!Deneris!
• Randy!Blakely!
• Ron!Emeson!
!
Project)Discussion:))Pat)Levitt)
Confirmed Attendees: (21)
Randy Blakely
Ron Emeson
Pat Levitt
Paul Gresch
Doug McMahon
Evan Deneris
Denise Malone
Ran Ye
Gregg Stanwood
Chris Svitek
Allison Limpert
Bruce Carter
Hideki Iwamoto
Jeremy Veenstra-VW
Justin Siemman
Richard O’Neil
Noah Green
Heidi Hamm
Kat Betke
Bing Zhang
Tommy Saborido
Regrets: (3)
Mary Michael-Woolman
Nicole Baganz
Mark Wallace
Don’t know (3)
Katia Belova
Gwynne Davis
Randi Ulbricht
Vanderbilt Silvio O. Conte Center for
Neuroscience Research
External Advisory Board
Susan Dymecki, Ph.D.
Lou Muglia, Ph.D.
Miklos Toth, Ph.D.
Administrative Core
Co-Director,
Mark Wallace, Ph.D.
Center Projects
Conte Center Cores
Pat Levitt
Ron Emeson
Molecular
Neuroanatomy
Administrative
Evan Deneris
Randy Blakely
Internal Advisory Board
Danny Winder, Ph.D.
Karoly Mirnics, Ph.D.
James Sutcliffe, Ph.D.
Center Director,
Randy Blakely, Ph.D.
Bioanalytical
Center for Molec
Neuroscience
Physiology and Behavior
Vanderbilt Brain
Institute
Bioinformatics &
Biostatistics
ES/Transgenic
Core
Genome Resources
Center
Neurochemistry
Core
Laboratory for
Neurobehavior
Vanderbilt
Resources
9/2/12%
Summary of Molecular Neuroanatomy Core
Juli Wu, Managing Director
The Molecular Neuroanatomy core will be able to receive request in approximately 2
months (November, 2012) for immunocytochemistry or in situ hybridization. Towards
this goal we are in the process of doing the following:
•
Hired Anddre Valdivia in July to serve as research technician. He has already
been trained to perform immunostaining, mouse transcardial perfusions, cryostat
sectioning, plasmid extraction, and transformations. Anddre is in the process of
training with Juli to gain expertise in working out protocols for optimization and
testing different antibodies. His last training module will be focused on gaining
expertise for performing and troubleshooting in situ hybridization.
•
The plan for the core tissue library is to have 4% PFA fixed tissues (using
C57BL6 mice) at E13, E16, E19/P0, P4, P7, P14, P21, >P30 for immunostaining
and in situ hybridization. A smaller collection of fresh frozen postnatal and adult
tissue will be generated for select in situ hybridization projects (for greater
sensitivity.
•
The MNAC can order a limited number of antibodies to test for your Conte project
if it is likely that extensive mapping will be needed. Investigators can also send
antibodies to the MNAC and the core will run pilot studies to optimize staining.
•
The Blakely Nlgn2 projects are serving as the core’s trial for in situ hybridization
training. In September, Anddre will make new Nlgn2 probes. Anddre will do initial
in situs with this probe.
•
Towards the end of year 01, Juli also will begin to introduce new in situ
hybridization techniques in order to examine the distribution of splice variants
and microRNAs. New methods are described in the literature, which Juli has
begun to adapt. [see Clovis et al, Development 139:3332 (2012) – Locked
Nucleic Acid (LNA) riboprobes for short sequence in situ hybridization; Baker,
Nature Methods 9:787 (2012) – short, singly labeled probes]
Clovis et al 2012 – MiR in situ (red) and FoxP2 immuno (green) combined
!
2012/2013!Conte!Meeting!Schedule!
Meetings(are(typically(on(Tuesday(evenings;((any(changes(made(to(the(schedule(will(be(immediately(
updated(on(the(online(calendar(and(forwarded(to(team(
!
• October!9,!2012:!!Evan!Deneris!
• November!2012:!!No!meeting,!Symposium!on!November!2!
• December!11,!2012:!!Randy!Blakely!
• January!15,!2013:!!Ronald!Emeson!
• February!19,!2013:!!Pat!Levitt!
• March!19,!2013:!!Evan!Deneris!
• April!16,!2013:!!Randy!Blakely!
• May!21,!2013:!!Ronald!Emeson!
• Summer!break:!!June,!July!and!August!2013!
!
!
2012!Conte!Symposium!Schedule,!
Friday,!November!2!
Plan(for(45(minutes(per(speaker,(including(Q&A(
sessions(
!
8:00!AM!!!
Brain!Matters!Tour!
9:00!AM!!
Laboratory!for!Neurobehavior!Tour!
10:00!AM!
Faculty!Meetings!with!Speakers!
• Holmes!with!Emeson!
• Ansorge!with!Blakely!
• Lin!with!Jeremy!VRVanderWeele!
• Gaspar!with!Deneris!
11:15!AM!!
Speaker!Lunch!at!University!Club!
• Randi!Ulbricht!
• Richard!O’Neil!
• Jeremy!Veenstra!VanderRWeele!
• Nicole!Baganz!
• Ran!Ye!
12:15!PM!
Escort!Speakers!to!Law!School!to!prepare!for!Symposium!
!
1:00!PM!
Symposium!Begins—Introductions!
1:10!PM!
Pat!Gaspar!
1:55!PM!
Rick!Lin!
2:40!PM!
Elizabeth!Hammock!
3:25!PM!
Reception!and!Poster!Session!
4:00!PM!
Mark!Ansorge!
4:45!PM!
Andrew!Holmes!
5:30!PM!
Closing!Remarks!
6:30!PM!
Faculty!Dinner!with!Speakers!
!
The Updated Conte Website
http://vandyconte.net, http://vandyconte.org or
http://www.mc.vanderbilt.edu/contecenter/
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New Features Include:
1. Functional tabs across the top of the website, consistent on every page; drop down menus
appear for subpages
2. Banner that will list the next Conte Meeting date and presenter
3. Events tab includes a traditional calendar with Conte events and other major meetings
4. Still in progress, but including all publications referencing Conte, organized by PI
Calendar Page showing Conte Center Events
(monthly meetings, annual symposium,
deadline for pilot grant applications, etc.)
Most events have clickable links to
corresponding websites
Example of pull down menus--under Pilot
Grants, you can view the recipients for each
year since the program’s inception. The
Symposia tab has options to review the info for
each of the previous years.
Goal is to have a registration page set up this
week for people to enroll for this year’s
symposium.