Product datasheet
Anti-HIF1 beta antibody [EPR17516] ab184711
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Overview
Product name
Anti-HIF1 beta antibody [EPR17516]
Description
Rabbit monoclonal [EPR17516] to HIF1 beta
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment within Human HIF1 beta aa 450 to the C-terminus. The exact sequence
is proprietary.
Database link: P27540
Run BLAST with
Run BLAST with
Positive control
WB: Human fetal kidney, pancreas and colon lysates; HeLa whole cell lysate. IHC-P: Human
uterus and hepatocellular carcinoma tissues.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR17516
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab184711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
IHC-P
Notes
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0
before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87
kDa).
Target
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding
subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then
initiates transcription of genes involved in the activation of PAH procarcinogens. The
heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive
response to hypoxia.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Cellular localization
Nucleus.
Anti-HIF1 beta antibody [EPR17516] images
Immunohistochemical analysis of paraffinembedded Human uterus tissue labeling HIF1
beta with ab184711 at 1/2000 dilution,
followed by Goat Anti-Rabbit IgG H&L (HRP)
(ab97051) secondary antibody at 1/500
dilution. Nuclear staining on epithelial cells of
Human uterus is observed. Counter stained
with Hematoxylin.
Secondary antibody only control: Used PBS
instead of primary antibody, secondary
Immunohistochemistry (Formalin/PFA-fixed
antibody is Goat Anti-Rabbit IgG H&L (HRP)
paraffin-embedded sections) - Anti-HIF1 beta
(ab97051) at 1/500 dilution.
antibody [EPR17516] (ab184711)
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Immunohistochemical analysis of paraffinembedded Human hepatocellular carcinoma
tissue labeling HIF1 beta with ab184711 at
1/2000 dilution, followed by Goat Anti-Rabbit
IgG H&L (HRP) (ab97051) secondary
antibody at 1/500 dilution. Nuclear staining
on cancer cells of hepatocellular carcinoma is
observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS
instead of primary antibody, secondary
Immunohistochemistry (Formalin/PFA-fixed
antibody is Goat Anti-Rabbit IgG H&L (HRP)
paraffin-embedded sections) - Anti-HIF1 beta
(ab97051) at 1/500 dilution.
antibody [EPR17516] (ab184711)
Anti-HIF1 beta antibody [EPR17516]
(ab184711) at 1/1000 dilution + Human fetal
kidney lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Predicted band size : 87 kDa
Observed band size : 87 kDa
Western blot - Anti-HIF1 beta antibody
[EPR17516] (ab184711)
Exposure time : 15 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-HIF1 beta antibody [EPR17516]
(ab184711) at 1/1000 dilution + HeLa
(Human epithelial cells from cervix
adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase
conjugated at 1/1000 dilution
Predicted band size : 87 kDa
Observed band size : 87 kDa
Western blot - Anti-HIF1 beta antibody
[EPR17516] (ab184711)
Exposure time : 8 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-HIF1 beta antibody
[EPR17516] (ab184711) at 1/2000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human colon lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Western blot - Anti-HIF1 beta antibody
[EPR17516] (ab184711)
Predicted band size : 87 kDa
Observed band size : 87 kDa
Exposure time : 3 minutes
Blocking/Dilution buffer: 5% NFDM/TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
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