Product datasheet
Rabbit IgG, monoclonal [EPR25A] - Isotype Control
(Phycoerythrin) ab209478
2 Images
Overview
Product name
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin)
Conjugation
Phycoerythrin. Ex: 488nm, Em: 575nm
Tested applications
ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Rabbit IgG, monoclonal - Isotype Control [EPR25A] (ab172730)
Rabbit IgG, monoclonal - Isotype Control (HRP) [EPR25A] (ab199507)
Rabbit IgG, monoclonal - Isotype Control (DyLight® 488) [EPR25A] (ab153686)
Rabbit IgG, monoclonal - Isotype Control (Alexa Fluor® 647) [EPR25A] (ab199093)
Rabbit IgG, monoclonal - Isotype Control (Alexa Fluor® 488) [EPR25A] (ab199091)
Rabbit IgG, monoclonal - Isotype Control [EPR25A] (Low endotoxin, Azide free) (ab209478)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C.
Do Not Freeze.
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR25A
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab209478 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
ICC/IF
Notes
1/500.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10
min) and 100% methanol (5 min)
Flow Cyt
Use at an assay dependent concentration.
Please note: This product should be diluted to the same concentration (not
dilution) of the primary antibody to be used.
Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) images
Overlay histogram showing HeLa cells
stained with ab208741 (red line). The cells
were fixed with 4% formaldehyde (10
min) and then permeabilized with 0.1% PBSTriton X-100 for 15 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum to block non-specific protein-protein
interactions followed by the antibody
(ab208741, 1/500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was
Flow Cytometry - Rabbit IgG, monoclonal
rabbit IgG monoclonal [EPR25A] (ab209478)
[EPR25A] - Isotype Control (ab209478)
used at the same concentration and
conditions as the primary antibody.
Unlabelled sample (blue line) was also used
as a control.
Acquisition of >5,000 events were collected
using a 20 mW Solid State Blue Laser
(488nm) and 585/40 bandpass filter.
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Immunofluorescent analysis of HeLa (human
cervical cancer) cells, fixed with 4%
formaldehyde (10 min). The cells were
permeabilized with 0.1% Triton X-100 for 5
minutes and then blocked with 1% BSA/10%
normal goat serum/0.3M glycine in 0.1%
PBS-Tween for 1h. The cells were then
incubated overnight at +4°C with ab209478
(Rabbit IgG, monoclonal [EPR25A] - Isotype
Control) at 1/500 dilution (showing no signal)
and ab195884, Rat monoclonal [YOL1/34] to
Immunocytochemistry/ Immunofluorescence -
Tubulin Microtubule Marker (Alexa Fluor®
Rabbit IgG, monoclonal [EPR25A] - Isotype
647), at 1/250 dilution (shown in red). Nuclear
Control (Phycoerythrin) (ab209478)
DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope
(Leica-Microsystems, TCS SP8).
This product also gave a positive signal under
the same testing conditions in HeLa cells
fixed with 100% methanol (5min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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