Blog | Abcam: ChIP protocols and new ChIP primers from Abcam
ChIP protocols and new ChIP primers from
Abcam
Posted At : October 9, 2009 6:01 PM | Posted By : Peter
Related Categories: Protocols, Website
Chromatin Immunoprecipitation (ChIP) enables the mapping of target proteins/histone
modifications to specific loci in the genome. Antibodies and protein A beads, which have a higher
affinity for some immunoglobulin isotypes, are used to immunoprecipitate chromatin/DNA
complexes. The DNA region of interest is determined using quantitative PCR.
Here are six protocols to help you get the best out of your experiment.
ChIP protocols
ChIP tips by Dr Andy Bannister (Gurdon institute)
Troubleshooting tips to ensure successful ChIP
ChIP using plant samples - Arabidopsis
Chromatin preparation from tissues for ChIP
Cross-linking chromatin immunoprecipitation (x-ChIP)
Native chromatin immunoprecipitation
If you're working in a different application, we now have over 70 protocols, including guides for
ELISA, FACS, IP and WB, and Abcam's Scientific support team are adding more all the time!
New ChIP primers - fast, easy, and consistent
We can now provide validated ChIP primers which you can use to:
Test antibodies for use in ChIP
Control for antibodies already validated in ChIP