Product datasheet
Anti-Catalase antibody ab1877
13 Abreviews 44 References 4 Images
Overview
Product name
Anti-Catalase antibody
Description
Rabbit polyclonal to Catalase
Tested applications
IHC-Fr, IHC-P, ICC, WB, IP, ELISA, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Human
Immunogen
Catalase [Bovine Liver]
Positive control
ICC: MDCK cells, COS-1 cells (see review) IHC-P: rat or mouse liver sections (see review) WB:
rat thoracic aorta and vena cava lysate (see review)
General notes
This product has been assayed against 1.0 ug of Catalase [Bovine liver] in a standard ELISA
using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room
temperature. A working dilution of 1:30,000 to 1:150,000 is suggested for this product. Storage:
Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge
product if not completely clear after standing at room temperature. This product is stable for
several weeks at 4° C as an undiluted liquid.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Purity
Preadsorbed IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step
process which includes delipidation, salt fractionation and ion exchange chromatography
followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis
resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially
purified Catalase [Bovine liver].
Clonality
Polyclonal
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab1877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
Use at an assay dependent dilution.
IHC-P
1/200 - 1/500. See Abreviews.
ICC
1/300.
WB
1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular
weight: 59 kDa).
IP
Use at an assay dependent concentration.
ELISA
1/60000 - 1/260000.
ICC/IF
1/300. See Abreviews. Fix in 4%PFA/20mM PIPES pH6.8/100mM NaCl/30mM
Glucose/2mM MgCl2/1mM EGTA.
Target
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic
effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid
leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in disease
Acatalasemia
Sequence similarities
Belongs to the catalase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Peroxisome.
Anti-Catalase antibody images
ab1877 at a dilution of 1/200 staining
Hydrogen peroxide treated HeLa cells (1mM,
1hr) by immunocytochemistry. The antibody
was incubated with the cells for 2 hours and
then detected using a Cy5 conjugated antirabbit antibody.
This image is courtesy of an Abreview
submitted by Jinhee Kim on 30 January
2006.
Immunocytochemistry/ Immunofluorescence Catalase antibody - Peroxisome Marker (ab1877)
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All lanes : Anti-Catalase antibody (ab1877)
at 1/5000 dilution
Lane 1 : 3ug liver 1 (Homogenate)
Lane 2 : 3ug liver 1 (PNS)
Lane 3 : 3ug liver 1 (Float 1)
Western blot - Catalase antibody - Peroxisome
Lane 4 : 3ug liver 1 (Float 2)
Marker (ab1877)
Lane 5 : 3ug liver 1(Interface - enriched
fraction)
Lane 6 : 3ug liver 2 (Homogenate)
Lane 7 : 3ug liver 2 (PNS)
Lane 8 : 3ug liver 2 (Float 1)
Lane 9 : 3ug liver 2 (Float 2)
Lane 10 : 3ug liver 2(Interface - enriched
fraction)
Secondary
HRP conjugated goat polyclonal antibody
developed using the ECL technique
Predicted band size : 59 kDa
Observed band size : 65 kDa
Additional bands at : <25 kDa (possible
degradation product).
Exposure time : 5 seconds
This image is courtesy of an anonymous
Abreview
developed using the ECL technique
Performed under reducing conditions.
Western blot - Catalase antibody - Peroxisome
Marker (ab1877)
Predicted band size : 59 kDa
Observed band size : 64 kDa
Exposure time : 3 minutes
This image is courtesy of an Abreview
submitted by Dr Rose Webster
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Catalase was immunoprecipitated using
0.5mg Hela whole cell extract, 5µg of Rabbit
polyclonal to Catalase (ab1877) and 50µl of
protein G magnetic beads (lane 1). The
antibody was incubated with the Protein G
beads for 10min under agitation. No antibody
was added to the control (lane 2). Hela whole
cell extract diluted in RIPA buffer was added
Immunoprecipitation - Anti-Catalase antibody -
to each sample and incubated for 10min
Peroxisome Marker (ab1877)
under agitation. Proteins were eluted by
addition of 40µl SDS loading buffer and
incubated for 10min at 70oC; 10µl of each
sample was separated on a SDS PAGE gel,
transferred to a nitrocellulose membrane,
blocked with 5% BSA and probed with
ab1877. Secodary: Mouse monoclonal
[SB62a] Secondary Antibody to Rabbit IgG
light chain (HRP) (ab99697).
Bands: 60kDa: Catalase
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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