Product datasheet
Anti-Lactate Dehydrogenase antibody [EP1566Y] (HRP)
ab202653
2 Images
Overview
Product name
Anti-Lactate Dehydrogenase antibody [EP1566Y] (HRP)
Description
Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase (HRP)
Conjugation
HRP
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Positive control
WB: HeLa whole cell lysate. IHC-P: FFPE human liver hepatocellular carcinoma tissue sections.
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
Anti-Lactate Dehydrogenase antibody (Alexa Fluor® 488) [EP1566Y] (ab202652)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Affinity Purified
Clonality
Monoclonal
Clone number
EP1566Y
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab202653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37
kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-Lactate Dehydrogenase antibody [EP1566Y] (HRP) images
2
IHC image of Lactate
Dehydrogenase staining in a section of
formalin-fixed paraffin-embedded human
hepatocellular carcinoma tissue*, performed
on a Leica BONDTM. The section was pretreated using heat mediated antigen retrieval
with sodium citrate buffer (pH6, epitope
retrieval solution 1) for 20mins. The section
was then incubated with ab202653, 1/50
dilution, for 15 mins at room temperature.
DAB was used as the chromogen. The
Immunohistochemistry (Formalin/PFA-fixed
section was then counterstained with
paraffin-embedded sections) - Anti-Lactate
haematoxylin and mounted with DPX. The
Dehydrogenase antibody [EP1566Y] (HRP)
inset negative control image is taken from an
(ab202653)
identical assay without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
3
Anti-Lactate Dehydrogenase antibody
[EP1566Y] (HRP) (ab202653) at 1/5000
dilution + HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate at 10 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 36 kDa
Western blot - Anti-Lactate Dehydrogenase
antibody [EP1566Y] (HRP) (ab202653)
Exposure time : 6 seconds
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 2% Bovine Serum Albumin before
being incubated with ab202653 overnight at
4°C. Antibody binding was visualised using
ECL development solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4