Product datasheet
Anti-Aldolase antibody [EPR9724(B)] (HRP) ab200774
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Overview
Product name
Anti-Aldolase antibody [EPR9724(B)] (HRP)
Description
Rabbit monoclonal [EPR9724(B)] to Aldolase (HRP)
Conjugation
HRP
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide corresponding to Human Aldolase.
Positive control
WB : A549 and HeLa whole cell lysates as well as Human fetal liver, fetal brain skeletal muscle
tissue lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Aldolase antibody [EPR9724(B)] (ab169544)
Anti-Aldolase antibody (Alexa Fluor® 488) [EPR9724(B)] (ab200771)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Purity
Affinity Purified
Clonality
Monoclonal
Clone number
EPR9724(B)
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab200774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/5000. Predicted molecular weight: 39 kDa.
Target
Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding
protein.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate
from D-glucose: step 4/4.
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881];
also known as red cell aldolase deficiency. A metabolic disorder associated with increased
hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and
rhabdomyolysis.
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family.
Anti-Aldolase antibody [EPR9724(B)] (HRP) images
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All lanes : Anti-Aldolase antibody
[EPR9724(B)] (HRP) (ab200774) at 1/5000
dilution
Lane 1 : A549 (Human lung adenocarcinoma
epithelial cell line) Whole Cell Lysate
Lane 2 : Fetal Liver (Human) Normal Tissue)
Lane 3 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 4 : Fetal Brain (Human) Normal Tissue
lysate
Western blot - Anti-Aldolase antibody
Lane 5 : Skeletal Muscle (Human) Tissue
[EPR9724(B)] (HRP) (ab200774)
Lysate - adult normal tissue (ab29330)
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 39 kDa
Observed band size : 39 kDa
Exposure time : 4 minutes
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 2% Bovine Serum Albumin before
being incubated with ab200774 overnight at
4°C. Antibody binding was visualised using
ECL development solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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