Product datasheet
Anti-alpha + beta Tubulin antibody [TU-10] ab30506
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Overview
Product name
Anti-alpha + beta Tubulin antibody [TU-10]
Description
Mouse monoclonal [TU-10] to alpha + beta Tubulin
Specificity
ab30506 recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of
microtubules.
Tested applications
WB, ICC
Species reactivity
Reacts with a wide range of species. Not yet tested in other species.
Immunogen
Microtubule proteins from porcine brain
Positive control
Porcine brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by precipitation methods and size-exclusion chromatography.
Clonality
Monoclonal
Clone number
TU-10
Isotype
IgM
Applications
Our Abpromise guarantee covers the use of ab30506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 2 µg/ml. Predicted molecular weight: 100 kDa. Use under
reducing conditions.
ICC
Use at an assay dependent dilution.
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Target
Relevance
Tubulin is the major building block of microtubules - an intracellular cylindrical filamentous
structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin
(100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides alpha
and beta. Alpha- and beta-tubulins are encoded by separate genes, or small gene families.
Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25
nm diameter. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge
from each other (both within and across species) at less than 10% of the amino acid positions,
the most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta
tubulins consist of various isotypes and both undergo different post-translational modifications.
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and
transport events to cell movement and the maintainance of cell shape.
Cellular localization
Cytoplasmic
Anti-alpha + beta Tubulin antibody [TU-10] images
Anti-alpha + beta Tubulin antibody [TU-10]
(ab30506) at 2 µg/ml + Human Jurkat T cell
line lysate (1% laurylmaltoside)
Secondary
Goat anti-mouse IgG (H+L)-HRP
Performed under non-reducing conditions.
Predicted band size : 100 kDa
Western blot - Anti-alpha + beta Tubulin antibody
[TU-10] (ab30506)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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