Product datasheet
Anti-EPLIN antibody ab154530
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Overview
Product name
Anti-EPLIN antibody
Description
Rabbit polyclonal to EPLIN
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment, corresponding to a region within amino acids 473-730 of Human EPLIN
(UniProt Q9UHB6).
Positive control
HeLa and HepG2 whole cell lysates; HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab154530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
ICC/IF
1/100 - 1/1000.
Target
Function
Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and
inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments,
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delays filament nucleation and reduces formation of branched filaments.
Tissue specificity
Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected
in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes.
Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells
from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial
cells and dermal fibroblasts. Not detectable in myocardium.
Sequence similarities
Contains 1 LIM zinc-binding domain.
Domain
Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains
bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than
the N-terminus.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein
co-localizes with actin stress fibers and focal adhesion plaques.
Anti-EPLIN antibody images
All lanes : Anti-EPLIN antibody (ab154530)
at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 85 kDa
Western blot - Anti-EPLIN antibody (ab154530)
Immunofluorescent analysis of methanol-fixed
HeLa cells labeling EPLIN with ab154530 at
1/500 dilution. Lower panel co-stained with
Hoechst 33342.
Immunocytochemistry/ Immunofluorescence Anti-EPLIN antibody (ab154530)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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Terms and conditions
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