Product datasheet
Anti-beta Actin antibody ab25894
5 References 3 Images
Overview
Product name
Anti-beta Actin antibody
Description
Rabbit polyclonal to beta Actin
Tested applications
ICC/IF, WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide. Ab25894 was raised against a 16 amino acid peptide from near the carboxyterminus of human ß-actin.
Positive control
HeLa cell lysate can be used as positive control.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab25894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 42 kDa
(predicted molecular weight: 42 kDa).
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
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Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are
ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of
dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle
contraction, often leading to abnormal postures. DYTJ patients manifest progressive,
generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing
loss.
Sequence similarities
Belongs to the actin family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated
mRNAs.
Anti-beta Actin antibody images
Predicted band size : 42 kDa
Western blot - beta Actin antibody (ab25894)
ab25894 at 10µg/ml staining beta Actin in
Hela cells by ICC/IF
Immunocytochemistry - beta Actin antibody
(ab25894)
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ab25894 (2µg/ml) staining beta actin in
human placenta.
Sections were stained using an automated
system (DAKO Autostainer Plus ), at room
temperature: sections were rehydrated and
antigen retrieved with the Dako 3 in 1 AR
buffers EDTA pH 9.0 . Slides were
peroxidase blocked in 3% H2O2 in methanol
for 10 mins. They were then blocked with
Immunohistochemistry (Formalin/PFA-fixed
Dako Protein block for 10 minutes (containing
paraffin-embedded sections)-beta Actin
casein 0.25% in PBS) then incubated with
antibody(ab25894)
primary antibody for 20 min and detected with
Dako envision flex amplification kit for 30
minutes. Colorimetric detection was
completed with Diaminobenzidine for 5
minutes. Slides were counterstained with
Haematoxylin and coverslipped under
DePeX. Please note that for manual staining
we recommend to optimize the primary
antibody concentration and incubation time
(overnight incubation), and amplification may
be required.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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