Product datasheet
Anti-LRP6 antibody ab24386
2 References 2 Images
Overview
Product name
Anti-LRP6 antibody
Description
Goat polyclonal to LRP6
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Dog, Chimpanzee
Immunogen
Synthetic peptide:
CMTSVATAKGYTSDL
, corresponding to amino acids 1546-1560 of Human LRP6
Run BLAST with
Epitope
Run BLAST with
This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human
LRP6 protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab24386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use at an assay dependent concentration.
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Target
Function
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through
inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cellsurface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation.
The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma
membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the
formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction
of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
Tissue specificity
Widely co-expressed with LRP5 during embryogenesis and in adult tissues.
Involvement in disease
Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2
(ADCAD2) [MIM:610947].
Sequence similarities
Belongs to the LDLR family.
Contains 4 EGF-like domains.
Contains 3 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats.
Domain
The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex.
The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to
activate the Wnt signaling pathway.
Post-translational
modifications
Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required
for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing
GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and
outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to
regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is
induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I
on Thr-1479.
Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The
extracellular domain is first released by shedding, and then, through the action of gammasecretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to
GSK3B and to activate canonical Wnt signaling.
Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from
the endoplasmic reticulum.
Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
N-glycosylation is required for cell surface location.
Cellular localization
Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrinmediated endocytosis and plasma membrane location. Released from the endoplasmic
reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the
absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with
AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma
membrane by MESD.
Anti-LRP6 antibody images
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Ab24386 (5ug/ml) staining human LRP6 in
human liver tissue by immunohistochemistry
using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer
pH 6, AP-staining.
In paraffin embedded Human Liver shows
strong cytoplasmic staining in hepatocytes
Immunohistochemistry (Paraffin-embedded
and cytoplasmic staining in bile ductulus cells.
sections) - LRP6 antibody (ab24386)
ab24386 staining LRP6 in Human Kidney at
4µg/ml by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
sections). Sections were subjected to
steamed antigen retrieval with citrate buffer
pH 9.0, and antibody was visualised with
Immunohistochemistry (Formalin/PFA-fixed
HRP-staining.
paraffin-embedded sections) - LRP6 antibody
(ab24386)
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