Product datasheet
Anti-FYB antibody [EP2546Y] ab76103
2 Abreviews 3 Images
Overview
Product name
Anti-FYB antibody [EP2546Y]
Description
Rabbit monoclonal [EP2546Y] to FYB
Tested applications
ICC/IF, WB, IHC-P, ICC, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
A synthetic peptide corresponding to residues on the N terminus of human FYB
Positive control
TF-1 lysate. Human tonsil tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Alternative versions available:
Anti-FYB antibody (Alexa Fluor® 488) [EP2546Y] (ab206707)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP2546Y
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab76103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
WB
IHC-P
ICC
Flow Cyt
Application notes
Flow Cyt: 1/60.
ICC: 1/100 - 1/250.
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
WB: 1/5000 - 1/10,000. Predicted molecular weight: 85 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the
expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of
SKAP1 and SKAP2.
Tissue specificity
Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not
expressed in B-cells, nor in non-lymphoid tissues.
Sequence similarities
Contains 1 SH3 domain.
Post-translational
modifications
T-cell receptor ligation leads to increased tyrosine phosphorylation.
Cellular localization
Cytoplasm. Nucleus.
Anti-FYB antibody [EP2546Y] images
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Anti-FYB antibody [EP2546Y] (ab76103) at
1/10000 dilution + TF-1 lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000
dilution
Predicted band size : 85 kDa
Observed band size : 110 kDa
Western blot - FYB antibody [EP2546Y]
(ab76103)
ab76103, at 1/250 dilution, staining FYB in
paraffin-embedded human tonsil tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - FYB antibody
[EP2546Y] (ab76103)
ICC/If image of CHO cell expressing human
ADAP using ab 76103. The cells were fixed
in formaldehyde and permeabilised using
0.2% Triton X100 for 5 mins. The cells were
them blocked using 10% serum for 1 hour.
Immunocytochemistry/ Immunofluorescence FYB antibody [EP2546Y] (ab76103)
Courtsey Anonymus Abreview
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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