Product datasheet
Anti-Dopamine antibody [2B11] ab1001
2 Abreviews 1 References 1 Image
Overview
Product name
Anti-Dopamine antibody [2B11]
Description
Mouse monoclonal [2B11] to Dopamine
Specificity
The affinity of this antibody for dopamine-BSA is 10 nM and for noradrenaline-BSA is 100 nM.
Affinity for unconjugated catecholamines(dopamine, noradrenaline, adrenaline) is lower (approx.
100 µM). At 1 uM, this antibody inhibits dopamine binding to membrane receptor from human
corpus striatum. Cross-reactivity (%): Dopamine = 100% Noradrenaline = <15% Adrenaline =
<10% Normetanephrine = 0% 3-methoxy-tyramine = 0% L-DOPA = 0% Homovanillic acid = 0%
Vanillic acid = 0% Vanillylmandelic acid = 0% 4-hydroxy-3-methoxyphenyl glycol = 0% 3,4dihydroxyphenylacetic acid = 0% Tyramine = 0% beta-phenylethylamine = 0% Amphetamine =
0%
Tested applications
Blocking, IHC-P, IHC-Fr
Species reactivity
Reacts with: Human
Immunogen
Full length protein corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA).
Immunogen = Dopamine conjugated to bovine serum albumin.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Purity
Protein A purified
Clonality
Monoclonal
Clone number
2B11
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab1001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Target
Relevance
Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its
chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the
hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the
pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart
rate and blood pressure.
Cellular localization
Secreted
Anti-Dopamine antibody [2B11] images
ab1001 staining human adrenal tissue
sections by IHC-P. Sections were
formaldehyde fixed and subjected to heat
mediated antigen retrieval in citrate buffer (pH
6) prior to blocking in 1% serum for 30
minutes at 22°C. The primary antibody was
diluted 1/1000 and incubated with the sample
for 30 minutes at 22°C. A HRP conjugated
goat anti-mouse antibody diluted 1/400 was
used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Dopamine antibody
(ab1001)
This image is courtesy of an anonymous Abreview
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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