Product datasheet
Anti-SCAMP1 antibody [EPR14493(B)] ab185951
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Overview
Product name
Anti-SCAMP1 antibody [EPR14493(B)]
Description
Rabbit monoclonal [EPR14493(B)] to SCAMP1
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human SCAMP1 aa 50-150. The exact sequence is proprietary.
Database link: O15126
Positive control
U87-MG, HeLa, Neuro-2a, Human fetal brain, C6 and RAW 264.7 lysates; HeLa cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR14493(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab185951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
1/1000 - 1/2000. Detects a band of approximately 38 kDa (predicted molecular
weight: 38 kDa).
Flow Cyt
1/160. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control
with this antibody.
Target
Function
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
Tissue specificity
Widely expressed, with highest expression in brain.
Sequence similarities
Belongs to the SCAMP family.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane.
Anti-SCAMP1 antibody [EPR14493(B)] images
Flow cytometric analysis of 2%
paraformaldehyde-fixed HeLa cells labeling
SCAMP1 with ab185951 at 1/160 dilution
(red) or a rabbit IgG isotype control (green),
followed by secondary Goat anti rabbit IgG
(FITC) at 1/150 dilution.
Flow Cytometry - Anti-SCAMP1 [EPR14493(B)]
antibody (ab185951)
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All lanes : Anti-SCAMP1 antibody
[EPR14493(B)] (ab185951) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Human fetal brain cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Western blot - Anti-SCAMP1 [EPR14493(B)]
Goat Anti-Rabbit IgG, (H+L), Peroxidase
antibody (ab185951)
conjugated at 1/1000 dilution
Predicted band size : 38 kDa
All lanes : Anti-SCAMP1 antibody
[EPR14493(B)] (ab185951) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
conjugated at 1/1000 dilution
Western blot - Anti-SCAMP1 [EPR14493(B)]
antibody (ab185951)
Predicted band size : 38 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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