Product datasheet
Anti-Delta Opioid Receptor antibody [EPR5029(2)]
ab176324
2 Images
Overview
Product name
Anti-Delta Opioid Receptor antibody [EPR5029(2)]
Description
Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P41143
Positive control
Human cerebellum, fetal brain, U87-MG, HUVEC and SH-SY5Y lysates. U87-MG cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated ‘PUR’ on our product labels.
If you have any questions regarding this update, please contact our Scientific Support
team.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR5029(2)
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab176324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
Relevance
The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for
enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa ,
delta, and mu) were first identified by their different selectivities for various naturally occurring
alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid
sequences of the opioid receptor subtypes are ~70% homologous, and are similar to
somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is
thought to occur at the third intracellular loop of the opioid receptors, which is also the location of
consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the
specific receptor subtypes are found on different chromosomes in both the human and mouse
genomes.
Cellular localization
Multi pass membrane protein
Anti-Delta Opioid Receptor antibody [EPR5029(2)] images
All lanes : Anti-Delta Opioid Receptor
antibody [EPR5029(2)] (ab176324) at 1/1000
dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : HUVEC lysate
Lane 5 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-Delta Opioid Receptor
[EPR5029(2)] antibody (ab176324)
Predicted band size : 40 kDa
2
Flow cytometric analysis of U87-MG cells
labeling Delta Opioid Receptor using
ab176324 at a 1/10 dilution (red) or a rabbit
IgG control (green).
Flow Cytometry - Anti-Delta Opioid Receptor
[EPR5029(2)] antibody (ab176324)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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