Product datasheet
Anti-Delta Opioid Receptor antibody ab63536
1 Abreviews 1 References 2 Images
Overview
Product name
Anti-Delta Opioid Receptor antibody
Description
Rabbit polyclonal to Delta Opioid Receptor
Specificity
This antibody detects endogenous levels of total Opioid Receptor delta protein.
Tested applications
WB, IHC-P, ELISA
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthesized non-phosphopeptide derived from human Delta Opioid Receptor around the
phosphorylation site of serine 363 (T-P-SP-D-G).
Positive control
COS7 cell lysate or human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab63536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular
weight: 40 kDa).
IHC-P
1/50 - 1/100.
ELISA
1/40000.
1
Target
Relevance
The opioid receptors are G-protein coupled, seven-transmembrane domain receptors for
enkephalins, endorphins, and dynorphins. Three different opioid receptor subtypes (kappa ,
delta, and mu) were first identified by their different selectivities for various naturally occurring
alkaloid opioid ligands, and subsequently confirmed by molecular cloning. The amino acid
sequences of the opioid receptor subtypes are ~70% homologous, and are similar to
somatostatin receptors (SSTRs) showing ~40 % homology with SSTR1. G-protein binding is
thought to occur at the third intracellular loop of the opioid receptors, which is also the location of
consensus sequences for phosphorylation of the receptor. Interestingly, the genes encoding the
specific receptor subtypes are found on different chromosomes in both the human and mouse
genomes.
Cellular localization
Multi pass membrane protein
Anti-Delta Opioid Receptor antibody images
All lanes : Anti-Delta Opioid Receptor
antibody (ab63536) at 1/500 dilution
Lane 1 : Extracts from COS7 cells (5-30ug),
without immunising peptide.
Lane 2 : Extracts from COS7 cells (5-30ug),
with immunising peptide (5-10ug).
Predicted band size : 40 kDa
Western blot - Delta Opioid Receptor antibody
Observed band size : 85 kDa
(ab63536)
Ab63536 (1/50-1/100) staining human Delta
Opioid Receptor in human brain tissue by
immunohistochemistry using formalin fixed,
paraffin embedded tissue.
The left hand panel is without immunising
peptide, whereas the right hand panel is in the
Immunohistochemistry (Formalin/PFA-fixed
presence of immunising peptide.
paraffin-embedded sections) - Delta Opioid
Receptor antibody (ab63536)
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Replacement or refund for products not performing as stated on the datasheet
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