Product datasheet
Anti-Cannabinoid Receptor I antibody [EPR2224(N)(B)]
ab172970
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Overview
Product name
Anti-Cannabinoid Receptor I antibody [EPR2224(N)(B)]
Description
Rabbit monoclonal [EPR2224(N)(B)] to Cannabinoid Receptor I
Tested applications
WB, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Cannabinoid Receptor I aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P21554
Positive control
Human fetal brain, HeLa and SH-SY5Y lysates; HeLa cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2224(N)(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab172970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular
weight: 19 kDa).
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for IHC-P or IP.
Target
Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a
receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have
altered ligand binding.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Anti-Cannabinoid Receptor I antibody [EPR2224(N)(B)] images
Immunofluorescent analysis of HeLa cells
labeling Cannabinoid Receptor I with
ab172970 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence Anti-Cannabinoid Receptor I [EPR2224(N)(B)]
antibody (ab172970)
Flow cytometric analysis of HeLa cells
labeling Cannabinoid Receptor I with
ab172970 (red) at 1/80 dilution compared to
a rabbit IgG (negative) (green).
ELISA - Anti-Cannabinoid Receptor I [EPR2224(N)
(B)] antibody (ab172970)
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All lanes : Anti-Cannabinoid Receptor I
antibody [EPR2224(N)(B)] (ab172970) at
1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : HeLa cell lysates
Lane 3 : SH-SY5Y lysates
Western blot - Anti-Cannabinoid Receptor I
Lysates/proteins at 10 µg per lane.
[EPR2224(N)(B)] antibody (ab172970)
Predicted band size : 19 kDa
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