Product datasheet
Anti-Phosphotyrosine antibody ab50722
2 Images
Overview
Product name
Anti-Phosphotyrosine antibody
Description
Rabbit polyclonal to Phosphotyrosine
Specificity
ab50722 recognizes tyrosine phosphorylated proteins. The antibody recognizes the pY-256
random peptide and phospho-L-tyrosine, but does not recognize phospho-L-serine, phosphoLthreonine or L-tyrosine.
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Species independent
Immunogen
Phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH.
Positive control
Total cell extract of A431 stimulated by human EGF
General notes
Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine
phosphorylation in many signal transduction pathways. Polyclonal antibodies can offer
advantages over monoclonals, especially in immunoprecipitation protocols, and can theoretically
react with a broader range of phosphotyrosine-containing proteins.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Purity
Immunogen affinity purified
Primary antibody notes
Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine
phosphorylation in many signal transduction pathways. Polyclonal antibodies can offer
advantages over monoclonals, especially in immunoprecipitation protocols, and can theoretically
react with a broader range of phosphotyrosine-containing proteins.
Clonality
Polyclonal
Isotype
IgG
Applications
1
Our Abpromise guarantee covers the use of ab50722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa.
ICC/IF
Use a concentration of 5 - 10 µg/ml.
Target
Relevance
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of
signal transduction during normal mitogenesis, cell cycle progression and oncogenic
transformation, its role in other areas such as differentiation and gap junction communication, is
a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated
tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins
and the biochemical pathways in which they function.
Anti-Phosphotyrosine antibody images
All lanes : Anti-Phosphotyrosine antibody
(ab50722) at 0.3 µg/ml
Lane 1 : Whole cell extracts of untreated
A431 cells
Lane 2 : Whole cell extracts of EGF-treated
A431 cells
Lane 3 : Whole cell extracts of
peroxyvanadate-treated A431 cells
Secondary
Western blot - Phosphotyrosine antibody
Goat Anti-Rabbit IgG-Peroxidase conjugate
(ab50722)
using a chemiluminescent substrate
developed using the ECL technique
Predicted band size : 10 kDa
Plasma membrane and cytoplasmic
staining A431 Cells were fixed with
paraformaldheyde and stained with ab50722
at 1/100, followed by Goat anti-Rabbit IgG
Immunofluorescence - Phosphotyrosine antibody
(H+L), FITC conjugate. Panel A: Serum-
(ab50722)
starved A431 cells stimulated with human
EGF (1 µg/mL, 5 min, EGF human). Panel B:
Untreated control A431 cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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