Product datasheet
Anti-CHX10 antibody [EPR7022] ab133636
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Overview
Product name
Anti-CHX10 antibody [EPR7022]
Description
Rabbit monoclonal [EPR7022] to CHX10
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to a region on the C terminal end of Human CHX10.
Positive control
Y79 cells and cell lysate, Fetal liver lysate
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 2.06 x 10 -10 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR7022
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab133636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular
weight: 39 kDa).
Flow Cyt
1/500 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an
isotype control with this antibody.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
Function
Plays a significant role in the specification and morphogenesis of the sensory retina. May also
participate in the development of the cells of the inner nuclear layer, particularly bipolar cells.
Tissue specificity
Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear
layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar
cells predominate.
Involvement in disease
Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also
known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder
of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular
tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and
choroid, cataractand other abnormalities like cataract may also be present.
Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities
(MCOPCTI) [MIM:610092].
Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3)
[MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a
set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the
fusion of the fetal fissure (optic fissure).
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 CVC domain.
Contains 1 homeobox DNA-binding domain.
Cellular localization
Nucleus.
Anti-CHX10 antibody [EPR7022] images
2
All lanes : Anti-CHX10 antibody [EPR7022]
(ab133636) at 1/1000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP conjugated antibody at
1/2000 dilution
Western blot - Anti-CHX10 antibody [EPR7022]
(ab133636)
Predicted band size : 39 kDa
Observed band size : 50 kDa
Flow cytometric analysis of permeabilized
Y79 cells labelling CHX10 with ab133636 at
1/500.
Flow Cytometry - Anti-CHX10 antibody [EPR7022]
(ab133636)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-CHX10 antibody [EPR7022](ab133636)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
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