Product datasheet
Anti-Vasopressin V1b receptor antibody ab104365
2 Images
Overview
Product name
Anti-Vasopressin V1b receptor antibody
Description
Rabbit polyclonal to Vasopressin V1b receptor
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse
Vasopressin V1b receptor.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue
lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab104365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa
(predicted molecular weight: 46 kDa).
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Application
Abreviews
ICC/IF
Notes
Use a concentration of 1 µg/ml.
Target
Function
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which
activate a phosphatidyl-inositol-calcium second messenger system.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Cellular localization
Cell membrane.
Anti-Vasopressin V1b receptor antibody images
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All lanes : Anti-Vasopressin V1b receptor
antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue
Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Membrane Lysate adult normal tissue (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic
fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue
Western blot - Anti-Vasopressin V1b receptor
Lysate with Immunising peptide at 1 µg/ml
antibody (ab104365)
Lane 6 : Forebrain (Mouse) Tissue Lysate
with Immunising peptide at 1 µg/ml
Lane 7 : Brain (Human) Membrane Lysate adult normal tissue (ab29456) with
Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic
fibroblast cell line) Whole Cell Lysate with
Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 49 kDa
Additional bands at : 102 kDa,22 kDa. We
are unsure as to the identity of these extra
bands.
Exposure time : 30 seconds
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ICC/IF image of ab104365 stained SKNSH
cells. The cells were 100% methanol fixed (5
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab104365, 1µg/ml)
overnight at +4°C. The secondary antibody
(green) was ab96899, DyLight® 488 goat
Immunocytochemistry/ Immunofluorescence -
anti-rabbit IgG (H+L) used at a 1/250 dilution
Anti-Vasopressin V1b receptor antibody
for 1h. Alexa Fluor® 594 WGA was used to
(ab104365)
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
This antibody also gave a positive result in
4% formaldehyde fixed (10 min) SKNSH cells
at 1µg/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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