Product datasheet
Anti-Sprouty 2 antibody [EPR4318(2)(B)] ab180527
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Overview
Product name
Anti-Sprouty 2 antibody [EPR4318(2)(B)]
Description
Rabbit monoclonal [EPR4318(2)(B)] to Sprouty 2
Tested applications
WB, IHC-P, ICC/IF, IP
Species reactivity
Reacts with: Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Sprouty 2 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: O43597
Positive control
HT-29, PC-3 and HeLa cell lysates; SH-SY5Y cells and cell lysates; Human breast carcinoma
tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR4318(2)(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab180527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular
weight: 35 kDa).
IHC-P
1/50.
ICC/IF
1/100.
IP
1/40.
Target
Function
May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively
modulate respiratory organogenesis.
Sequence similarities
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain.
Domain
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in
unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF.
Anti-Sprouty 2 antibody [EPR4318(2)(B)] images
Predicted band size : 35 kDa
Western blot analysis of Hela cell lysate
immunoprecipitated with ab180527.
Detection utilised ab180527 at 1/40 dilution
followed by Goat Anti-Rabbit IgG, (H+L),
Peroxidase conjugated at 1/1000 dilution.
The blocking buffer and dilution buffer was 5%
NFDM/TBST.
Western blot - Anti-Sprouty 2 [EPR4318(2)(B)]
antibody (ab180527)
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Immunofluorescence analysis of
paraformaldehyde-fixed SH-SY5Y cells
labeling Sprouty 2 with ab180527 at 1/1000
dilution. Goat anti-rabbit IgG (Dylight 488) at
1/250 dilution was used as the secondary
antibody. The slide is counterstained with
Dapi.
Immunocytochemistry/ Immunofluorescence Anti-Sprouty 2 [EPR4318(2)(B)] antibody
(ab180527)
Immunohistochemical analysis of paraffinembedded Human breast carcinoma tissue
labeling Sprouty 2 with ab180527 at 1/50
dilution. The slide is counterstained with
Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Sprouty 2
[EPR4318(2)(B)] antibody (ab180527)
All lanes : Anti-Sprouty 2 antibody
[EPR4318(2)(B)] (ab180527) at 1/10000
dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
Western blot - Anti-Sprouty 2 [EPR4318(2)(B)]
conjugated at 1/1000 dilution
antibody (ab180527)
Predicted band size : 35 kDa
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All lanes : Anti-Sprouty 2 antibody
[EPR4318(2)(B)] (ab180527) at 1/2000
dilution
Lane 1 : HT-29 cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
Western blot - Anti-Sprouty 2 [EPR4318(2)(B)]
conjugated at 1/1000 dilution
antibody (ab180527)
Predicted band size : 35 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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