Product datasheet
Anti-MAP1B antibody [AA6] ab11266
1 Abreviews 6 References 1 Image
Overview
Product name
Anti-MAP1B antibody [AA6]
Description
Mouse monoclonal [AA6] to MAP1B
Specificity
Monoclonal Anti-MAP1b does not react with tubulin of other Microtubule associated proteins.
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
Immunogen
Rat brain microtubule associated proteins.
Positive control
Rat brain.
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged
storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Purity
Ascites
Clonality
Monoclonal
Clone number
AA6
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab11266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ICC/IF
Application notes
ICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or
immunoperoxidase labelling methods.
WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation
1
sites: 320kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Microtubules are associated with a family of proteins called microtubule associated proteins
(MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2,
MAP3, MAP4 and MAP5. MAP1B is the major microtubule associated protein in developing
brain which changes its expression during development. In the newborn rat brain, it is a major
component of microtubules but in the adult its level is ten fold lower. This change in the level of
expression occurs simultaneously with neuronal maturation. MAP1B is the first MAP to appear in
growing axons during development as it is present from the first emergence of the nascent axon
from the cell body.
Cellular localization
Cytoplasmic
Anti-MAP1B antibody [AA6] images
ab11266 (1/300) staining MAP1B (centre
column) in Human cortical neurons by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% paraformaldehyde.
Left column staining: tubulin, actin, synapsin,
SAP102. Right column: overlay.
Immunocytochemistry/ Immunofluorescence Anti-MAP1B antibody [AA6] (ab11266)
Image from Eriksson M et al., Neurosci Lett, 475(1),
33-7. Fig 2.; doi: 10.1016/j.neulet.2010.03.039. with
permission from Elsevier.
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