Product datasheet
Anti-Laminin antibody (Biotin) ab6571
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Overview
Product name
Anti-Laminin antibody (Biotin)
Description
Rabbit polyclonal to Laminin (Biotin)
Conjugation
Biotin
Specificity
Negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific
cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin
extracellular matrix proteins is negligible.
Tested applications
ELISA, IHC-P, IP, WB, IHC-Fr
Species reactivity
Reacts with: Rat, Human
Predicted to work with: all Mammals
Immunogen
Full length protein (Human).
General notes
This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
Purity
IgG fraction
Purification notes
This product has been prepared by immunoaffinity chromatography using immobilized antigens
followed by extensive cross-adsorption against human serum proteins and collagen and noncollagen extracellular matrix proteins to remove any unwanted specificities. Typically less than
1% cross reactivity against other extracellular matrix proteins was detected by ELISA against
purified standards.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab6571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
ELISA
1/20000 - 1/100000.
IHC-P
1/1000 - 1/5000.
IP
1/100.
WB
1/2000 - 1/10000.
IHC-Fr
1/500. PubMed: 17251454
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment,
migration and organization of cells into tissues during embryonic development by interacting with
other extracellular matrix components.
Tissue specificity
Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a
coiled coil structure.
Domain IV is globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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