Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-Ephrin A1 antibody [EPR1786(2)]
Rabbit monoclonal [EPR1786(2)] to Ephrin A1
WB
Reacts with: Human
Synthetic peptide corresponding to a region within Human Ephrin A1.
HUVEC cell lysate; HUVEC cell lysate treated with TNF alpha
This product is a recombinant rabbit monoclonal antibody.
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Clone number
Isotype
Liquid
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Monoclonal
EPR1786(2)
IgG
Applications
Our Abpromise guarantee covers the use of ab133598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
WB
Application notes
Target
Function
Tissue specificity
Sequence similarities
Post-translational modifications
Cellular localization
Abreviews Notes
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Plays an important role in angiogenesis and tumor neovascularization. The recruitement of
VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of
EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by downregulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
Belongs to the ephrin family.
Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
Secreted and Cell membrane.
Anti-Ephrin A1 antibody [EPR1786(2)] images
All lanes : Anti-Ephrin A1 antibody
[EPR1786(2)] (ab133598) at 1/1000 dilution
Lane 1 : HUVEC cell lysate treated with TNF alpha
Lane 2 : HUVEC cell lysate
Western blot - Anti-Ephrin A1 antibody
[EPR1786(2)] (ab133598)
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 24 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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