Product datasheet
Anti-Brevican antibody ab67580
1 Image
Overview
Product name
Anti-Brevican antibody
Description
Mouse polyclonal to Brevican
Tested applications
WB
Species reactivity
Reacts with: Human
Immunogen
Full length Brevican protein (Human)
Positive control
Brevican transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab67580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: 1/500 - 1/1000. Detects bands of approximately 125 and 170 kDa (predicted molecular
weight: 99 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
1
Relevance
Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the
adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans
that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and
is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes
and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is
also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular
glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains
the unique C-terminal epitope EAMESE.
Cellular localization
Isoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the
membrane; Lipid-anchor, GPI-anchor.
Anti-Brevican antibody images
All lanes : Anti-Brevican antibody (ab67580)
at 1/500 dilution
Lane 1 : Brevican transfected 293T cell
lysate
Lane 2 : Non-transfected 293T cell lysate
Western blot - Brevican antibody (ab67580)
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
at 1/2500 dilution
Predicted band size : 99 kDa
Observed band size : 170 kDa
Additional bands at : 125 kDa. We are
unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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Terms and conditions
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