Product datasheet
Anti-EMR3 antibody ab115460
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Overview
Product name
Anti-EMR3 antibody
Description
Rabbit polyclonal to EMR3
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to the C Terminal cytoplasmic domain of
Human EMR3.
Positive control
Human Macrophages.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -80°C.
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab115460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Relevance
EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family expressed
predominantly by cells of the immune system. Family members are characterized by an
extended extracellular region with a variable number of N-terminal epidermal growth factor
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(EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is
closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2
on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune
and inflammatory responses. Alternative splicing of this gene results in two transcript variants
encoding two distinct isoforms. EMR3 has been reported to be expressed in neutrophils,
monocytes, and macrophages. ESTs have been isolated from liver/spleen and mixed libraries.
Cellular localization
Cell membrane; Multi-pass membrane protein. EMR3 subunit alpha: Secreted, extracellular
space. Note: A soluble form is also detected.
Anti-EMR3 antibody images
ab115460 at 2.5 µg/ml staining EMR3 in
Formalin-fixed, Paraffin-embedded Human
Macrophages by Immunohistochemistry
followed by biotinylated secondary antibody,
alkaline phosphatase-streptavidin and
chromogen.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-EMR3 antibody
(ab115460)
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