Product datasheet
Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC)
ab24885
1 References 1 Image
Overview
Product name
Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC)
Description
Mouse monoclonal [74-22-15] to Monocyte + Granulocyte (FITC)
Conjugation
FITC. Ex: 493nm, Em: 528nm
Specificity
This antibody reacts with >90% of pig monocytes and granulocytes and <5% of peripheral blood
lymphocytes.
Tested applications
Flow Cyt, IP, IHC-Fr
Species reactivity
Reacts with: Pig
Immunogen
dd miniature swine thymocytes
General notes
This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Purity
IgG fraction
Primary antibody notes
This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.
Clonality
Monoclonal
Clone number
74-22-15
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab24885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1
Application
Abreviews
Notes
Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as
Flow Cyt
an isotype control with this antibody.
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Target
Relevance
A monocyte is a leukocyte, part of the human body's immune system that protects against bloodborne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues.
Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Granulocytes
are a category of white blood cells characterised by the presence of granules in their cytoplasm.
They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying
shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term
polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most
abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the
regulatory complement proteins.
Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC) images
Fresh heparinized blood from a healthy adult
pig was stained with ab24885 at 1 µg/106
cells. Red blood cells were lyzed with
ammonium chloride and the cells were then
fixed in 1% paraformaldehyde. Granulocytes
were gated and analyzed on a FACScanTM
flow cytometer (BDIS, San Jose, CA).
Flow Cytometry - Monocyte + Granulocyte
antibody [74-22-15] (FITC) (ab24885)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
2
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3