Product datasheet
Anti-IFN gamma Receptor beta antibody [MMHGR-2]
ab25448
1 Abreviews 1 References 1 Image
Overview
Product name
Anti-IFN gamma Receptor beta antibody [MMHGR-2]
Description
Mouse monoclonal [MMHGR-2] to IFN gamma Receptor beta
Specificity
ab25448 binds to but does not neutralise human interferon gamma receptor.
Tested applications
WB, IP, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Human interferon gamma receptor beta.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1% BSA, PBS
Clonality
Monoclonal
Clone number
MMHGR-2
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab25448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/2000. Predicted molecular weight: 38 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
Application notes
Is unsuitable for Neut.
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Target
Function
Part of the receptor for interferon gamma. Required for signal transduction. This accessory
factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact
with GAF, JAK1, and/or JAK2.
Involvement in disease
Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD)
[MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare
condition confers predisposition to illness caused by moderately virulent mycobacterial species,
such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous
mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms
rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections,
with the exception of Salmonella which infects less than 50% of these individuals. The
pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated
immunity, whose severity determines the clinical outcome. Some patients die of overwhelming
mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop,
later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a
genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked
inheritance.
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Cellular localization
Membrane.
Anti-IFN gamma Receptor beta antibody [MMHGR-2] images
Overlay histogram showing Ramos cells
stained with ab25448 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-IFN gamma Receptor beta
antibody [MMHGR-2](ab25448)
(ab25448, 1µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in Ramos
cells fixed with 4% paraformaldehyde (10
min)/permeabilized with 0.1% PBS-Tween for
20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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