Product datasheet
Anti-CD68 antibody [ED1] ab31630
11 Abreviews 35 References 4 Images
Overview
Product name
Anti-CD68 antibody [ED1]
Description
Mouse monoclonal [ED1] to CD68
Specificity
Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on
the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The
antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood
granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of
human CD68.
Tested applications
Flow Cyt, IHC-Fr, IP, WB, RIA, IHC-FrFl, IHC-P
Species reactivity
Reacts with: Mouse, Rat
Immunogen
Tissue/ cell preparation: Rat spleen cells
General notes
This product should be stored undiluted.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein A purified
Clonality
Monoclonal
Clone number
ED1
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab31630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
1
Application
Abreviews
Flow Cyt
Notes
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with
this antibody.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
RIA
Use at an assay dependent concentration.
IHC-FrFl
Use at an assay dependent concentration. PubMed: 25374513
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC
staining protocol. Alternatively protein digestion with trypsin or pronase prior to
IHC protocol could be preformed.
Target
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal
metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organspecific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid
recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow
macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes,
fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to
attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similarities
Belongs to the LAMP family.
Post-translational
modifications
N- and O-glycosylated.
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane.
Anti-CD68 antibody [ED1] images
2
Staining of rat peritoneal macrophages
withAlexa Fluor®647 conjugated Mouse anti
Rat CD68, clone ED1 (ab31630) following
permeabilisation with Leucoperm.
Flow Cytometry - Anti-CD68 antibody [ED1]
(ab31630)
Immunofluorescence analysis of rat lymph
node labelling CD 68 with ab31630. DAPI
was used, staining DNA. A green CD4
antibody was used to counterstain. The image
was taken at a low magnification. A is an
Image of DAPI & ab31630, B is an image of
Immunocytochemistry/ Immunofluorescence -
the CD4 counterstain & DAPI, C is merged
Anti-CD68 antibody [ED1] (ab31630)
showing the CD4 counterstain, DAPI &
ab31630.
ab31630 staining CD68 in rat liver tissue
sections by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
sections). Tissue was fixed with formaldehyde
and a heat mediated antigen retrieval step
was performed using Citrate buffer pH6.
Samples were then blocked with 1% BSA for
10 minutes at 21°C followed by incubation
with the primary antibody at a 1/800 dilution,
Immunohistochemistry (Formalin/PFA-fixed
for 2 hours at 21°C. A biotin-conjugated goat
paraffin-embedded sections) - CD68 antibody
anti-mouse IgG polyclonal was used as
[ED1] (ab31630)
secondary antibody at a 1/200 dilution.
Image courtesy of an Abreview submitted by Dr. Carl
Hobbs, King`s College London, United Kingdom
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ab31630 staining CD68 in rat kidney tissue
sections by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
sections). Tissue was fixed with formaldehyde
and a heat mediated antigen retrieval step
was performed using Citrate buffer. Samples
were then blocked with 10% serum for 1 hour
at 4°C followed by incubation with the primary
antibody at a 1/200 dilution, for 12 hours at
Immunohistochemistry (Frozen sections) - CD68
antibody [ED1] (ab31630)
Image courtesy of an anonymous Abreview.
4°C. A biotin-conjugated goat anti-mouse IgG
polyclonal was used as secondary antibody at
a 1/100 dilution.
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