Overview
Product name
Description
Specificity
Tested applications
Species reactivity
Immunogen
Anti-ICAM1 (phospho Y512) antibody
Rabbit polyclonal to ICAM1 (phospho Y512)
The antibody detects endogenous levels of ICAM-1 only when phosphorylated at tyrosine 512.
WB, ELISA
Reacts with: Human
The antiserum was produced against synthesised phosphopeptide derived from ICAM-1 around
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab51033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 96 kDa (predicted molecular
ELISA weight: 58 kDa).
1/40000.
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Target
Function
Sequence similarities
Post-translational modifications
Cellular localization
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Membrane.
Anti-ICAM1 (phospho Y512) antibody images
All lanes : Anti-ICAM1 (phospho Y512) antibody (ab51033) at 1/500 dilution
Lane 1 : Hela cells treated with TNF alpha with immunizing peptide
Lane 2 : Hela cells treated with TNF alpha
Predicted band size : 58 kDa
Observed band size : 96 kDa
Western blot - ICAM1 (phospho Y512) antibody
(ab51033)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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