Product datasheet
Anti-CD1a antibody [7A7] ab113618
7 Images
Overview
Product name
Anti-CD1a antibody [7A7]
Description
Mouse monoclonal [7A7] to CD1a
Tested applications
WB, ELISA, IHC-P, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Purified recombinant fragment of Human CD1a expressed in E. Coli.
Positive control
K562, Raji and MOLT4 cell lysates; Human cervical cancer, colon cancer, submaxillary tumor
and brain tissues; Raji cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.03% Sodium azide
Constituent: 99% Ascites
Purity
Ascites
Clonality
Monoclonal
Clone number
7A7
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab113618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 37 kDa.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
ICC/IF
1/200 - 1/1000.
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Application
Abreviews
Flow Cyt
Notes
1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype
control with this antibody.
Target
Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents
them to T-cell receptors on natural killer T-cells.
Tissue specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell
leukemias, and in various other tissues.
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell
membrane and endosomes. Localizes to cell surface lipid rafts.
Anti-CD1a antibody [7A7] images
All lanes : Anti-CD1a antibody [7A7]
(ab113618) at 1/500 dilution
Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MOLT 4 cell lysate
Predicted band size : 37 kDa
Western blot - CD1a antibody [7A7] (ab113618)
ab113618 at 1/200 dilution staining CD1a in
Paraffin-embedded Human cervical cancer
tissue by Immunohistochemistry followed by
DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CD1a antibody
[7A7] (ab113618)
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ab113618 at 1/200 dilution staining CD1a in
Paraffin-embedded Human colon cancer
tissue by Immunohistochemistry followed by
DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CD1a antibody
[7A7] (ab113618)
ab113618 at 1/200 dilution staining CD1a in
Paraffin-embedded Human brain tissue by
Immunohistochemistry followed by DAB
staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CD1a antibody
[7A7] (ab113618)
ab113618 at 1/200 dilution staining CD1a in
Paraffin-embedded Human submaxillary
tumor tissue by Immunohistochemistry
followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CD1a antibody
[7A7] (ab113618)
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Overlay histogram showing MOLT4 cells
stained with ab113618 (red line). The cells
were fixed with 80% methanol (5 min) and
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions. The cells were
then incubated with the antibody (ab113618,
1/100 dilution) for 30 min at 22ºC. The
Flow Cytometry-Anti-CD1a antibody [7A7]
secondary antibody used was DyLight® 488
(ab113618)
goat anti-mouse IgG (H+L) (ab96879) at
1/500 dilution for 30 min at 22ºC. Isotype
control antibody (black line) was mouse IgG1
[ICIGG1] (ab91353, 2µg/1x106 cells) used
under the same conditions. Acquisition of
>5,000 events was performed. This antibody
gave a positive result in 4%
paraformaldehyde (10 min) fixed MOLT4 cells
used under the same conditions.
Please note that Abcam do not have any data
for use of this antibody on non-fixed cells. We
welcome any customer feedback.
ab113618 at 1/200 dilution staining CD1a in
Raji cells by Immunofluorescence (green).
DRAQ5 fluorescent DNA dye (blue).
Immunocytochemistry/ Immunofluorescence CD1a antibody [7A7] (ab113618)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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