Product datasheet
Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S]
(Biotin) ab25229
4 References
Overview
Product name
Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Biotin)
Description
Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd + H2 Dd (Biotin)
Conjugation
Biotin
Specificity
ab25229 binds to a common determinant in the alpha3 domain of H2Kd and H2Dd in the
presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H2Kb.
Tested applications
Flow Cyt, IHC-Fr, Functional Studies
Species reactivity
Reacts with: Mouse
Immunogen
C3H.SW mouse splenocytes
Epitope
ab25229 recognizes an epitope in the alpha3 domain that is common to H2Kd and H2Dd.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Purity
IgG fraction
Clonality
Monoclonal
Clone number
34-1-2S
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab25229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
1
Application
Abreviews
Notes
Use 1µl for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as
Flow Cyt
an isotype control with this antibody.
IHC-Fr
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration. Complement-mediated cytotoxicity
Target
Relevance
The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene
complex and consist of heterodimers of highly polymorphic alpha chains noncovalently
associated with the invariant beta 2-microglobulin. These antigens are expressed on most
nucleated cells but expression varies on different cell types. MHC Class I molecules present
endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.
MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative
selection of CD8+ T cells during T cell ontogeny.
Cellular localization
Type I membrane protein
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S]