Product datasheet
Anti-Bcl3 antibody [1E8] ab49470
1 Abreviews 1 References 3 Images
Overview
Product name
Anti-Bcl3 antibody [1E8]
Description
Mouse monoclonal [1E8] to Bcl3
Tested applications
ICC/IF, IHC-P, IHC-Fr, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Recombinant protein corresponding to a 200 amino acid N terminal truncate of the Bcl-3
molecule.
Positive control
Immunohistochemistry: Tonsil.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
1E8
Myeloma
P3-NS1/1-Ag4-1
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab49470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/25 - 1/50.
IHC-Fr
1/100. Acetone fixation is recommended.
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Application
Abreviews
Notes
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as
Flow Cyt
an isotype control with this antibody.
Target
Function
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the
cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts
as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes
to the regulation of cell proliferation.
Involvement in disease
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic
leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
Sequence similarities
Contains 7 ANK repeats.
Post-translational
modifications
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear
accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation.
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin
chains is required for nuclear accumulation.
Anti-Bcl3 antibody [1E8] images
ab49470, at a dilution of 1/25, staining Bcl3
oncoprotein in paraffin sections of normal
human tonsil by immunohistochemistry. Note
nuclear staining of a proportion of follicular
cells, parafollicular cells and
mucosa.ab49470, at a dilution of 1/25,
Immunohistochemistry (Paraffin-embedded
staining Bcl3 oncoprotein in paraffin sections
sections) - Bcl3 antibody [1E8] (ab49470)
of normal human tonsil by
immunohistochemistry. Note nuclear staining
of a proportion of follicular cells, parafollicular
cells and mucosa.
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ICC/IF image of ab49470 stained MCF7
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab49470, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
ab96879, a goat anti-mouse DyLight® 488
(IgG; H+L) used at a 1/250 dilution for 1h.
Immunocytochemistry/ Immunofluorescence-Bcl3
antibody [1E8](ab49470)
Alexa Fluor® 594 WGA was used to label
plasma membranes (red) at a 1/200 dilution
for 1h. DAPI was used to stain the cell nuclei
(blue) at a concentration of 1.43µM.
Overlay histogram showing Ramos cells
stained with ab49470 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions followed by the
Flow Cytometry-Anti-Bcl3 antibody [1E8]
(ab49470)
antibody (ab49470, 1µg/1x106 cells) for 30
min at 22ºC. The secondary antibody used
was a goat anti-mouse DyLight® 488 (IgG;
H+L) (ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG2a [ICIGG2A] (ab91361,
1µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed.
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