Product datasheet
Anti-ASTL antibody ab59889
1 Image
Overview
Product name
Anti-ASTL antibody
Description
Rabbit polyclonal to ASTL
Specificity
We have a range of domain specific antibodies for this target. For a full list please see all ASTL
antibodies. ab59889 recognizes the propeptide domain in the zymogen, and not the cleaved
active catalytic domain.
Tested applications
WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide based on the propeptide domain of human ASTL. Immunizing peptide
available on request - please contact a member of staff to ask for availability.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab59889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 46 kDa.
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric
substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Function
Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a
role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage
of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and
cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.
Tissue specificity
Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in
some ovarian carcinomas. Not detected in normal tissues.
Sequence similarities
Belongs to the peptidase M12A family.
Cellular localization
Cytoplasm. Cell membrane. Cytoplasmic granule. Cytoplasmic vesicle > secretory vesicle.
Secretory granules. Localizes to the peripheral cortical granules of ovulated eggs. Probably
exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of
germinal vesicle stage oocytes in early bilaminar secondary follicles shortly after birth. Detected
in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first
polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS).
Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline
space (PVS).
Anti-ASTL antibody images
All lanes : Anti-ASTL antibody (ab59889) at
1/1000 dilution
Lane 1 : Tissue lysate prepared from rat
ovary (5 mg/ml) at 35 µl
Lane 2 : Tissue lysate prepared from rat
ovary (5 mg/ml) at 20 µl
Lane 3 : Tissue lysate prepared from rat
ovary (5 mg/ml) at 10 µl
Lane 4 : Tissue lysate prepared from rat
Western blot - ASTL antibody - Propeptide
domain (ab59889)
ovary (5 mg/ml) at 5 µl
Lane 5 : Tissue lysate prepared from rat
ovary (5 mg/ml) at 2 µl
Lane 6 : Tissue lysate prepared from mouse
ovary(5 mg/ml) at 35 µl
Lane 7 : Tissue lysate prepared from mouse
ovary (5 mg/ml) at 20 µl
Lane 8 : Tissue lysate prepared from mouse
ovary (5 mg/ml) at 10 µl
Lane 9 : Tissue lysate prepared from mouse
ovary (5 mg/ml) at 5 µl
Lane 10 : Tissue lysate prepared from
mouse ovary (5 mg/ml) at 2 µl
Predicted band size : 46 kDa
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