Product datasheet
Anti-Neprilysin-like Protease beta antibody ab59449
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Overview
Product name
Anti-Neprilysin-like Protease beta antibody
Description
Rabbit polyclonal to Neprilysin-like Protease beta
Specificity
This antibody recognises forms of Neprilysin-like Protease beta of 835, 779, 777, 770, 762 and
753 amino acids in length. We have a range of domain specific antibodies for this target. For a
full list please see all Neprilysin-like Protease beta antibodies.
Tested applications
WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide based on the propeptide domain of human Neprilysin-like Protease beta.
General notes
Immunizing peptide available on request – please contact a member of staff to ask for
availability.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab59449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for
chemiluminescent substrates. Predicted molecular weight: 110 kDa.
Glycosylation and other posttranslational modifications make human Neprilysin-like Protease
beta run slightly higher on reduced Western blots.
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Neprilysin like Proteases (alpha, beta, gamma) arise from the alternative splicing of a single
Neprilysin like Protease gene and show ~54% sequence homology. M13 members are
generally type II transmembrane proteins consisting of a single polypeptide chain with Zinc
binding HEXXH motif, a short cytoplasmic tail, a transmembrane segment and an extra
cytoplasmic domain containing the enzyme active site. Neprilysin like Protease beta is type II
transmembrane enzyme containing a single polypeptide chain of 765aa (~110kD) with
cytoplasmic and transmembrane domains and a large extracellular C terminal core containing
the peptidase active site. In comparison to Neprilysin like Protease alpha, Neprilysin like
Protease beta has an additional and unique sequence of 23 aa (41-63 aa) after the TM domain.
There are two splice variants of Neprilysin like Protease beta; a secreted isoform of 126kD
containing a 23 aa secretion signal sequence and a membrane associated isoform of 110kD.
The secreted isoform is much more glycosylated than the membrane isoform. A number of
different sequences for human Neprilysin like Protease beta are reported, with 835, 779, 777,
770, 762 and 753 amino acids in length, having predicted masses of 94.8, 89.4, 89.0, 88.5,
87.2 and 86.2 kDa. The 835 amino acid form has a longer and different carboxytermnius than
the other forms. The 753 amino acid form has a 26 residue deletion in the juxtamembrane
extracellular region, relative to the other forms, and the 770 form starts at the methionine
downstream 10 residues from the other forms. The 762 form uses a different exon in the
propeptide domain, and a 17 residue deletion. All of these forms have intact catalytic sites, and
are thought to be active enzymes.
Cellular localization
Membrane; Single pass type II membrane protein. Secreted.
Anti-Neprilysin-like Protease beta antibody images
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All lanes : Anti-Neprilysin-like Protease beta
antibody (ab59449) at 1/1000 dilution
Lane 1 : Recombinant Human Neprilysin-2 at
0.04 µg
Lane 2 : Recombinant Human Neprilysin-2 at
0.02 µg
Lane 3 : Recombinant Human Neprilysin-2 at
0.01 µg
Western blot - Neprilysin-like Protease beta
Lane 4 : Recombinant Human Neprilysin-2 at
antibody - Propeptide domain (ab59449)
0.005 µg
Lane 5 : Recombinant Human Neprilysin-1 at
0.04 µg
Lane 6 : Recombinant Human Neprilysin-1 at
0.02 µg
Lane 7 : Recombinant Human Neprilysin-1 at
0.01 µg
Lane 8 : Recombinant Human Neprilysin-1 at
0.005 µg
Predicted band size : 110 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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Anti-Neprilysin-like Protease beta antibody ab59449