Product datasheet
Anti-FISH antibody ab118575
1 References 2 Images
Overview
Product name
Anti-FISH antibody
Description
Goat polyclonal to FISH
Tested applications
IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide:
C-KPIEKSQFIHNNLK
, corresponding to amino acids 1033-1046 of Human FISH (NP_055446.2).
Run BLAST with
Positive control
Run BLAST with
Human Liver, and Small Intestine tissues
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab118575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 3.75 - 7.5 µg/ml.
Target
Function
Adapter protein involved in invadopodia and podosome formation, extracellular matrix
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degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs),
NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows
NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In
association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide.
Tissue specificity
Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.
Sequence similarities
Belongs to the SH3PXD2 family.
Contains 1 PX (phox homology) domain.
Contains 5 SH3 domains.
Domain
The PX domain is required for podosome localization because of its ability to bind
phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate
(PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P),
phosphatidylinositol 5-biphosphate (PtdIns(5)P), and phosphatidylinositol 3,5-biphosphate
(PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain.
The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.
Post-translational
modifications
Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein
localization. The intramolecular interaction of the PX domain with the third SH3 domain
maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in
the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on
serine upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Cell projection > podosome. Cytoplasmic in normal cells and localizes to
podosomes in SRC-transformed cells.
Anti-FISH antibody images
Human Liver: Formalin-Fixed, ParaffinEmbedded (FFPE) using ab118575 at a
concentration of 7.5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-FISH antibody
(ab118575)
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Human Small Intestine: Formalin-Fixed,
Paraffin-Embedded (FFPE) using ab118575
at a concentration of 7.5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-FISH antibody
(ab118575)
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