Product datasheet
Anti-CBR1 antibody [EPR9661(B)] ab174852
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Overview
Product name
Anti-CBR1 antibody [EPR9661(B)]
Description
Rabbit monoclonal [EPR9661(B)] to CBR1
Tested applications
WB, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Cynomolgus Monkey, Orangutan
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human CBR1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P16152
Positive control
293T, HeLa, MCF-7 and HepG2 cell lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR9661(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab174852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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Application
Abreviews
IP
Application notes
Notes
1/10 - 1/100.
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
Function
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide
variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various
xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and
daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert
prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher
affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localization
Cytoplasm.
Anti-CBR1 antibody [EPR9661(B)] images
Western blot analysis on immunoprecipitation
pellet from (1) HeLa cells lysate or (2) 1XPBS
(negative control) using ab174852, and
HRPconjugated anti-rabbit IgG preferentially
detecting the non-reduced form of rabbit IgG.
Immunoprecipitation - Anti-CBR1 [EPR9661(B)]
antibody (ab174852)
All lanes : Anti-CBR1 antibody
[EPR9661(B)] (ab174852) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-CBR1 [EPR9661(B)] antibody
(ab174852)
Predicted band size : 30 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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