Product datasheet
Anti-Proteasome 20S alpha 2 antibody [MCP21]
ab22666
1 Abreviews 1 References 3 Images
Overview
Product name
Anti-Proteasome 20S alpha 2 antibody [MCP21]
Description
Mouse monoclonal [MCP21] to Proteasome 20S alpha 2
Specificity
ab22666 reacts with 20S proteasome subunit a2 (HC3).
Tested applications
Flow Cyt, WB, IP, Other
Species reactivity
Reacts with: Rabbit, Human
Does not react with: Mouse, Rat
Immunogen
Full length protein corresponding to Human Proteasome 20S alpha 2.
Database link: P25787
Positive control
human placental proteasome preparation and HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.01M Sodium Azide
Constituents: PBS
Purity
IgG fraction
Clonality
Monoclonal
Clone number
MCP21
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab22666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
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Application
Abreviews
Notes
WB
1/1000. Detects a band of approximately 27 kDa.
IP
Use at an assay dependent concentration.
AP
Use at an assay dependent concentration.
Other
Use at an assay dependent concentration.
Target
Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to
cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or
slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have
a potential regulatory effect on another component(s) of the proteasome complex through
tyrosine phosphorylation.
Sequence similarities
Belongs to the peptidase T1A family.
Post-translational
modifications
Phosphorylated on tyrosine residues; which may be important for nuclear import.
Cellular localization
Cytoplasm. Nucleus.
Anti-Proteasome 20S alpha 2 antibody [MCP21] images
Luminograph of human placental proteasome
preparation after 2D PADE followed by
blotting onto nitrocellulose and probing with
ab22666 (1/1000). ECL exposure , 1 minute
exposure).
Electrophoretic Mobility Shift Assay - Proteasome
20S alpha 2 antibody [MCP21] (ab22666)
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All lanes : Anti-Proteasome 20S alpha 2
antibody [MCP21] (ab22666) at 1/1000
dilution
Lane 1 : 40ug SU-DHL1 whole cell lysate
Lane 2 : 40ug Karpas 299 whole cell lysate
Lane 3 : 40ug JB-6 whole cell lysate
Lane 4 : 40ug SUP-M2 whole cell lysate
Western blot - Proteasome 20S alpha 2 antibody
[MCP21] (ab22666)
Lane 5 : 40ug Mac-1 whole cell lysate
Lane 6 : 40ug Jurkat whole cell lysate
Secondary
Biotinylated polyclonal anti-mouse antibody.
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 27 kDa
Exposure time : 1 minute
This image is courtesy of an Abreview
submitted by Dr Carlo Vigliani
Overlay histogram showing HeLa cells
stained with ab22666 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-Proteasome 20S alpha 2
(ab22666, 1µg/1x106 cells) for 30 min at
antibody [MCP21](ab22666)
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
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