Product datasheet
Anti-MAT tag antibody [MAT1-87] ab50292
Overview
Product name
Anti-MAT tag antibody [MAT1-87]
Description
Mouse monoclonal [MAT1-87] to MAT tag
Specificity
ab50292 recognizes the seven amino acid MAT epitope sequence within either N or C terminal
MAT-tagged fusion proteins
Tested applications
ICC/IF, WB, IP, ELISA
Immunogen
A synthetic MAT peptide
HNHRHKHGGGC
conjugated to KLH.
Run BLAST with
Positive control
Run BLAST with
Cell extracts expressing a C-terminal MAT-tagged protein.
General notes
Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno
detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and
purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Purity
IgG fraction
Purification notes
Purified immunoglobulin
Primary antibody notes
Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno
detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and
purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.
Clonality
Monoclonal
Clone number
MAT1-87
Isotype
IgG2a
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Applications
Our Abpromise guarantee covers the use of ab50292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 1 kDa.
IP
Use a concentration of 5 - 10 µg/ml.
5 - 10 µg can immunoprecipitate a recombinant C-terminal MAT-tagged protein.
ELISA
Use at an assay dependent concentration.
Target
Relevance
The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel
and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT
fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly
selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both
prokaryotic and eukaryotic expression vectors.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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