Product datasheet
Anti-STK38 antibody [6F1] ab125131
5 Images
Overview
Product name
Anti-STK38 antibody [6F1]
Description
Mouse monoclonal [6F1] to STK38
Tested applications
Sandwich ELISA, WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fragment, corresponding to C terminal amino acids 365-465 of Human STK38
with 26kDa proprietary tag (AAH12085).
Positive control
Human Testis tissue; PC12 and NIH 3T3 cell lysates; HeLa nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.20
Constituent: 99% PBS
Purity
Ascites
Clonality
Monoclonal
Clone number
6F1
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab125131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Sandwich ELISA
Use at an assay dependent concentration.
WB
1/500 - 1/1000. Predicted molecular weight: 54 kDa.
IHC-P
Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
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Target
Function
Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to
its nonphosphorylated form and inhibits autophosphorylation of MAP3K2.
Tissue specificity
Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Post-translational
modifications
ISGylated.
Cellular localization
Nucleus. Cytoplasm.
Anti-STK38 antibody [6F1] images
ab125131, at 10µg/ml, staining STK38 in
formalin-fixed, paraffin-embedded Human
testis tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-STK38
antibody [6F1] (ab125131)
Anti-STK38 antibody [6F1] (ab125131) +
PC12 cell lysate
Predicted band size : 54 kDa
Western blot - Anti-STK38 antibody [6F1]
(ab125131)
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Anti-STK38 antibody [6F1] (ab125131) +
Hela nuclear extract
Predicted band size : 54 kDa
Western blot - Anti-STK38 antibody [6F1]
(ab125131)
Anti-STK38 antibody [6F1] (ab125131) + NIH
3T3 cell lysate
Predicted band size : 54 kDa
Western blot - Anti-STK38 antibody [6F1]
(ab125131)
Using ab125131 as a capture antibody, the
detection limit for recombinant proprietary
tagged STK38 is approximately 0.1ng/ml.
Sandwich ELISA - Anti-STK38 antibody [6F1]
(ab125131)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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