Product datasheet
Anti-HGF antibody [24612.111] ab10678
3 References
Overview
Product name
Anti-HGF antibody [24612.111]
Description
Mouse monoclonal [24612.111] to HGF
Specificity
The product shows no cross reactivity with other tested cytokines using indirect ELISA.
Tested applications
ELISA, WB, Neutralising
Species reactivity
Reacts with: Human
Immunogen
Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
Positive control
Purchase matching WB positive control:
Recombinant human HGF protein
General notes
Endotoxin: <5 ng/vial by LAL method.
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a
pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer
cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes
and other epithelial and endothelial cells to various biological actions, including mitogenic,
morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: PBS
Purity
Protein A purified
Purification notes
The antibody is purified using Protein A affinity chromatography.
Primary antibody notes
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a
pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer
cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes
and other epithelial and endothelial cells to various biological actions, including mitogenic,
morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
Clonality
Monoclonal
Clone number
24612.111
1
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab10678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ELISA
Abreviews
Notes
Use a concentration of 0.5 - 1 µg/ml.
Use at 0.5 - 1 µg/ml to detect <1.2 ng/well of rhHGF.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83.1 kDa.
Use at 1 - 2 µg/ml to detect rhHGF at 50 ng/lane under non-reducing conditions.
Neutralising
Use a concentration of 0.01 - 0.5 µg/ml.
Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity
of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell
line responsive to HGF. The ND50 of the antibody is defined as the concentration
of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is
present at a concentration just high enough to elicit a maximum response. In this
bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the
antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells
were added to each well and incubated for 48 hours at 37°C in a 5% CO2
humidified incubator and then pulsed for the last 24 hours with 3H-thymidine. Cells
were harvested onto glass filters and the 3H-thymidine incorporation into DNA
was measured.
Target
Function
HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an
hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It
has no detectable protease activity.
Involvement in disease
Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39)
[MIM:608265]. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness
results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or
the area of the brain that receives sound information.
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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