Product datasheet
Anti-p57 Kip2 antibody ab4058
3 Abreviews 12 References 5 Images
Overview
Product name
Anti-p57 Kip2 antibody
Description
Rabbit polyclonal to p57 Kip2
Specificity
This antibody reacts with a 57 kDa protein.
Tested applications
WB, ICC/IF, IHC-P, IHC-Fr
Species reactivity
Reacts with: Mouse, Cow, Human
Immunogen
Synthetic peptide (Human).
Positive control
Colon Carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab4058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa
(predicted molecular weight: 32 kDa).
ICC/IF
Use at an assay dependent concentration. PubMed: 17709399
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen
retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Application
Abreviews
IHC-Fr
Notes
Use at an assay dependent concentration.
Target
Function
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative
regulator of cell proliferation. May play a role in maintenance of the non-proliferative state
throughout life.
Tissue specificity
Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are
seen in the placenta while low levels are seen in the liver.
Involvement in disease
Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650].
BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects
including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia.
Additional less frequent complications include specific developmental defects and a
predisposition to embryonal tumors.
Note=Defects in CDKN1C are involved in tumor formation.
Sequence similarities
Belongs to the CDI family.
Cellular localization
Nucleus.
Anti-p57 Kip2 antibody images
Tissues were from mouse E14.5 wild-type
embryonic heads fixed in neutral buffered
formalin sectioned (5-7 um) horizontally
through the eyes. Antigen retrieval (0.1M
citrate buffer pH6.5) in a 1400W microwave
(5 min high; 5 min low). Sections blocked in
0.4% BSA/5% goat serum in PBS for 1h.
Antibody diluted (1:100) in block soln, applied
overnight at 4C. Detection with anti-Rb Alexa
green Ab (1:500), nuclear counterstain with
Hoechst dye (bisbenzimide).
Immunohistochemistry (Formalin-fixed paraffinembedded sections) - p57 Kip2 antibody (ab4058)
Taken from an Abreview submitted by Robb de
Longh. We do not have any further information
relating to this image.
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ab4058 staining mouse E14.5 embryonic eye
tissue sections by IHC-Fr. Sections were
PFA fixed and blocked with 1% BSA for 2
hours at 25°C prior to incubating with ab4058,
diluted 1/100, for 12 hours at 25°C. A FITC
conjugated goat anti-rabbit antibody, diluted
1/400 was used as the secondary.
Immunohistochemistry (Frozen sections) - p57
Kip2 antibody (ab4058)
This image is courtesy of an Abreview submitted by
Dr Bing Lang
ab4958 staining p57 Kip2 in non-transgenic
mouse embryos by Immunohistochemistry
(PFA fixed, paraffin embedded sections).
Heads of mice were removed, fixed in 10%
formalin, dehydrated, embedded in paraffin,
sectioned (5-7 µm).
Slides containing ocular sections from nonImmunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - p57 Kip2 antibody
(ab4058)
Image from Dr D Burgess et al, BMC Dev Biol. 2010
Jan 27;10:13, Fig 8.
transgenic embryos were first deparaffinized
and rehydrated. Antigens were retrieved by
microwave treatment in 10 mM Sodium
Citrate buffer (pH 6.0). Following antigen
retrieval, the tissue sections were blocked
with 10% normal horse serum for 30 minutes,
at room temperature. The slides were then
incubated with ab4058 at a 1/250 dilution
overnight at 4°C. Following brief washes in
PBS, the slides were incubated with the
appropriate biotinylated-secondary
antibodies for 30 minutes at 37°C. Antigenantibody complexes were then detected using
streptavidin-linked Alexa 594 (red) at 1/1000
dilution. Sections were mounted using antifade media containing DAPI (blue). Images
were captured using a Nikon Eclipse
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Anti-p57 Kip2 antibody (ab4058) at 1 µg/ml +
Skeletal Muscle (Human) Tissue Lysate adult normal tissue (ab29330) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to
Rabbit IgG - H&L (HRP), pre-adsorbed at
1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-p57 Kip2 antibody (ab4058)
Predicted band size : 32 kDa
Observed band size : 57 kDa
Additional bands at : 32 kDa,51 kDa. We
are unsure as to the identity of these extra
bands.
Exposure time : 3 minutes
Immunohistochemical analysis of formalinfixed, paraffin-embedded Human placenta
tissue, staining p57 Kip2 with ab4058.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-p57 Kip2
antibody (ab4058)
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