Product datasheet
Anti-Mkl1 antibody ab49311
1 Abreviews 2 References 2 Images
Overview
Product name
Anti-Mkl1 antibody
Description
Rabbit polyclonal to Mkl1
Specificity
This antibody is specific for Mkl1.
Tested applications
ICC/IF, IP, WB, ELISA
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
Immunogen
Synthetic peptide: QPLSQPGFPA PGPPAQMDLE HPPQPPFATP TSLLKKEPPG
YEETVTQQPk, corresponding to amino acids 721-770 of Mouse Mkl1
Run BLAST with
Positive control
Run BLAST with
NIH3T3 lysate. MEF1 cell line
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab49311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 10 µg/ml.
IP
Use at an assay dependent concentration. PubMed: 23243284
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Application
Abreviews
WB
Notes
Use a concentration of 1.25 µg/ml. Detects a band of approximately 109 kDa
(predicted molecular weight: 99 kDa). Good results were obtained when blocked
with 5% non-fat dry milk in 0.05% PBS-T.
ELISA
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:1562500.
Target
Function
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF
target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its
transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate
antiapoptotic molecules, which in turn inhibit caspase activation.
Tissue specificity
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney,
spleen, thymus, colon, muscle, heart and brain.
Involvement in disease
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic
leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion
transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the
candidate oncogene.
Sequence similarities
Contains 2 RPEL repeats.
Contains 1 SAP domain.
Domain
The N-terminal region is required for nuclear localization and the C-terminal region mediates
transcriptional activity.
The RPEL repeats mediate binding to globular actin.
Cellular localization
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
Anti-Mkl1 antibody images
Anti-Mkl1 antibody (ab49311) at 1.25 µg/ml +
NIH3T3 lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000
dilution
Predicted band size : 99 kDa
Observed band size : 109 kDa
Western blot - Mkl1 antibody (ab49311)
2
ICC/IF image of ab49311 stained MEF1
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab49311, 10µg/ml)
overnight at +4°C. The secondary antibody
(green) was ab96899, DyLight® 488 goat
anti-rabbit IgG (H+L) used at a 1/250 dilution
Immunocytochemistry/ Immunofluorescence -
for 1h. Alexa Fluor® 594 WGA was used to
Anti-Mkl1 antibody (ab49311)
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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